An 11.76-kilobase-pair (kb) segment of DNA from Bradyrhizobium sp. (Vigna) strain IRc 78 that hybridizes to nodulation genes of Rhizobium meliloti strain 41 was isolated. Hybridization of the 11.76-kb DNA fragment to DNA from other Bradyrhizobium species revealed a high degree of sequence conservation in this region. Transfer of the 11.76-kb segment to nodulation-defective (Nod(-)) mutants of R...

Flavonoids secreted by legumes play a major role as signal molecules for attracting compatible rhizobia. The aim of this study was to assess and understand the diversity of microsymbionts nodulating Bambara groundnut (Vigna subterranea L. Verdc.) landraces of different seedcoat colours using restriction fragment length polymorphism and phylogenetic analysis. Seedcoat pigmentation of landraces h...

Bambara groundnut (Vigna subterranea (L.) Verdc.) [BGN] is an easy-to-cultivate legume seed classified under the family Fabaceae, sub-family Faboidea and genus Vigna [1]. Two botanical varieties exist, namely V. subterranea var. spontanea (wild varieties) and V. subterranea var. subterranea (cultivated varieties). BGN originated in West Africa from the Bambara district near Timbuktu and is now ...

Cowpea ( Vigna unguiculata (L.) Walp) is an important legume crop for food security in areas of low-input and smallholder farming throughout Africa and Asia. Genetic improvements are required to increase yield and resilience to biotic and abiotic stress and to enhance cowpea crop performance. An integrated cowpea genomic and gene expression data resource has the potential to greatly accelerate ...

The soils of tropical tasar plantation fields in general are sloppy and are primarily deficient of nutrition due to top soil erosion and constant leaf productivity exploitation to rear tasar silk insect, Antheraea mylitta Drury. The rainfed field of Terminalia tomentosa (W&A) plantation has been evaluated for soil fortification with green mulching through legumes, Sun hemp (Crotalaria juncea), ...

Two hundred and thirty six soil samples from farmers’ fields in the Gumla and Saraikela districts of Jharkhand, and Jhabua and Mandla districts of Madhya Pradesh were analyzed for soil chemical fertility. Results showed deficiencies of sulphur (43 to 100% of samples), boron (69 to 98%), and zinc (5 to 73%). Majority of the farmers’ fields, except for those in the Saraikela district, fell in the...

The adzuki bean (Vigna angularis (Ohwi) Ohwi and Ohashi) is an important grain legume of Asia. It is cultivated mainly in China, Japan and Korea. Despite its importance, few genomic resources are available for molecular genetic research of adzuki bean. In this study, we developed EST-SSR markers for the adzuki bean through next-generation sequencing. More than 112 million high-quality cDNA sequ...

An attempt was made to identify the role of peroxidase activity as well as the changes of peroxidase (POX) isozymes that control adventitious root formation at the base of hypocotyl cuttings of mung bean (Vigna radiata L. cv. 105). Three phases of the adventitious root formation process were identified; induction (0-24 h), initiation (24-72 h) and expression (after 72 h). The lower peroxidase a...

Hydrogenperoxide (H(2)O(2)) is an end product of diamine and polyamine oxidation by their respective oxidase enzymes. A new sensitive assay method is based on a H(2)O(2)-titanium (Ti) complex formation as an indicator of H(2)O(2) production due to polyamine oxidation. The orange-yellow coloured H(2)O(2)-Ti complex was measured at 410 nm in a Shimadzu spectrophotometer. The assay conditions for ...

A well saturated genomic map is a necessity for a breeding program based on marker assisted selection. To this end, we are developing genomic maps for cowpea (Vigna unguiculata 2N = 22) and mung bean (Vigna radiata 2N = 22) based on restriction fragment length polymorphism (RFLP) markers. Using these maps, we have located major quantitative trait loci (QTLs) for seed weight in both species. Two...