Coagulation is triggered during the onset of myocardial infarction, resulting in vascular occlusion. However, a causal role for individual haemostatic factors in the development of thrombotic occlusion is not established. Three cases (all relatively young women) are reported of raised factor VIII associated with myocardial infarction. Two patients presented acutely with myocardial infarction at...

A trace level concentration of osmium(VIII) has been quantitatively extracted from 1.0 M hydrochloric acid with 0.087 M N-n-octylaniline as a stationery phase on silica gel. Osmium(VIII) has been eluted with 5.5 M, 25 mL hydrochloric acid and determined by spectrophotometric method. Various parameters influencing the extraction, such as acid concentration, reagent concentration and flow rate ha...

When purified antihemophilic factor (Factor VIII) was rechromatographed on 4% agarose in 0.15 M NaCl or 1.0 M NaCl, a single protein peak, containing both procoagulant activity and von Willebrand factor activity, as defined by ristocetin-induced platelet aggregation, was eluted in the void volume. Purified Factor VIII immediately lost about 30% of its procoagulant activity when dissolved in 0.2...

Penelitian ini dilatarbelakangi dengan kurangnya kemampuan menulis teks puisi siswa SMP Negeri 2 tigapanah, hal terlihat dari hasil belajar masih rendah atau dibawah standar kriteria ketuntasan KKM. menggunakan pendekatan kuantitatif desain penelitian pre-tes dan pos-tes, dalam populasi adalah kelas VIII jumlah 114, terdiri empat yaitu VIII-1, VIII-2,VIII-3, VIII-4. Maka peneliti menentukan sam...

An 82-year-old woman presented with severe bleeding due to antibodies inactivating both human and porcine factor VIII. Treatment with porcine factor VIII was successful in correcting the hemorrhagic manifestations, despite the fact that subsequent studies showed that the baseline porcine inhibitor titer was greater than the human inhibitor titer. Anamnestic response to porcine factor VIII did n...

normal VIII-related protein, as described by Holmberg and Nilsson (1973). The three patients to whom cryoprecipitate was given all showed a postinfusion correction of Ristocetin aggregation, although the response of factor VIII and VIII-related protein varied in each case. To the standard definition of classical von Willebrand's disease, it is now possible to add abnormal Ristocetin-induced agg...

Prophylactic treatment for hemophilia A involves infusion of factor VIII (FVIII) concentrates every 2 to 3 days. Liposomes can be efficacious vehicles for medicines, and surface modification by PEGylation can prolong liposome circulation time. When reconstituted with PEGylated liposomes (PEGLip's), recombinant FVIII binds noncovalently but with high affinity to the external liposome surface. Th...

Anti-factor VIII (FVIII) antibodies is a major complication of FVIII replacement therapy for hemophilia A. We investigated the immune response to recombinant human factor VIII Fc (rFVIIIFc) in comparison to BDD-rFVIII and full-length rFVIII (FL-rFVIII) in hemophilia A mice. Repeated administration of therapeutically relevant doses of rFVIIIFc in these mice resulted in significantly lower antibo...

The complement protein C4b-binding protein plays an important role in the regulation of the protein C anticoagulant pathway. C4b-binding protein can bind to protein S, thereby inhibiting the cofactor activity of protein S for activated protein C. In this report, we describe a new role for C4b-binding protein in coagulation. We observed inhibition of the intrinsic factor X activating reaction by...

Apparently quiescent, nonapoptotic endothelial cells mediate the activation of factor X by activated factor IX in the presence of its cofactor, activated factor VIII. In a previous study, we reported that during the activation of factor X, the interaction of the cofactor with the endothelial cell membrane clearly differs from the interaction of the cofactor with artificial lipid membranes. In t...