Poly(N-vinylimidazole), PVIm, gels were prepared by gamma-irradiation polymerization of N-vinylimidazole in aqueous solutions as an affinity gel for glucose oxidase (GOx). These affinity gels with a water swelling ratio of 1800% for plain polymeric gel and between 30-80% for Cu(II) and Co(II)-chelated gels at pH 6.0 in phosphate buffer were used in the GOx adsorption studies. Maximum metal ion ...

In our study, we aimed to synthesize novel grafted hyaluronic acid with cationic biodegradable polymer, poly (N-vinyl imidazole) (PVI), through free radical polymerization using potassium persulfate as initiator. The effect of various grafting factors including initiator and monomer concentrations, reaction time and temperature was studied on the percentage of grafting parameters such as; graft...

Molecular imprinting is one of the most efficient methods for preparing synthetic receptors that possess user defined recognition properties. Despite general success of non-covalent imprinting for a large variety of templates, some groups of compounds remain difficult to tackle due to their structural complexity. In this study we investigate preparation of molecularly imprinted polymers that ca...

Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic NMR spectroscopy. Several strategies have been proposed to site-specifically tag proteins with paramagnetic lanthanide ions. Here we report a simple approach of engineering a transition metal binding motif via site-specific labelling ...

’ The reductive debxomination of BrCCls by ferrous deoxymyoglobin Ieads to the covalent bonding of the prosthetic heme to the protein. We have previously shown, by the use of peptide mapping and mass spectrometry, that histidine residue 93 is covalently bound to the heme moiety. In the present study the structure of the heme adduct was more campletely determined by ’H and 13C NMIl techniques. W...

Resonance Raman spectroscopy is employed to characterize heme site structural changes arising from conformational heterogeneity in deoxyMb and ligated derivatives, i.e., the ferrous CO (MbCO) and ferric cyanide (MbCN) complexes. The spectra for the reversed forms of these derivatives have been extracted from the spectra of reconstituted samples. Dramatic changes in the low-frequency spectra are...

The handling of vinyl polysiloxane (addition silicone) impression putties with latex gloves is said to interfere with the setting of these impression materials. The aim of this study is to evaluate the effect of handling techniques on the setting of vinyl polysiloxane impression putties using several types of gloves. The setting of these materials was evaluated by means of an elasticimeter. Fou...

This work reports experimental and theoretical first-order rate constants for the reaction of vinyl radical with C(4)H(8) alkenes: 1-butene, 2-butene, and isobutene. The experiments are performed over a temperature range of 300 to 700 K at 100 Torr. Vinyl radicals (H(2)C horizontal lineCH) were generated by laser photolysis of vinyl iodide (C(2)H(3)I) at 266 nm, and time-resolved absorption spe...

Until now, acrylates have been the monomers of choice for use for step and flash imprint lithography ~SFIL! etch barrier formulations, in part because of the commercial availability of silicon-containing acrylates ~necessary for etch resistance!, together with their low viscosities and capability for rapid photopolymerization. However, despite many desirable properties, the polymerization of ac...

Vinyl flooring manufacturers use plasticizers to decrease the viscosity and increase the pliability of vinyl. Several ignitable liquid plasticizers used in the manufacture of vinyl flooring were identified and investigated in this study. Twenty-nine collections from five major vinyl manufacturers, a total of 72 samples, were analyzed using passive headspace concentration in accordance with the ...