The completion of human genome sequencing has shifted the focus of research from genes to proteins. In this regard, a protein library chip has become a useful tool for cell-free protein synthesis. In this study, we attempted to make a highly-integrated protein chip from a DNA library using in vitro protein synthesis on a microchamber array fabricated by using PDMS (polydimethyl siloxane), a hyd...

Chromatin immunoprecipitation (ChIP-chip) experiments enable capturing physical interactions between regulatory proteins and DNA in vivo. However, measurement of chromatin binding alone is not sufficient to detect regulatory interactions. A detected binding event may not be biologically relevant, or a known regulatory interaction might not be observed under the growth conditions tested so far. ...

BACKGROUND Leber congenital amaurosis (LCA) is the most severe form of inherited retinal visual impairment in children. So far, mutations in more than 20 genes have been known to cause LCA and among them, RPE65 is a suitable candidate for gene therapy. The mutational screenings of RPE65 and other LCA genes are requisite in support of emerging gene specific therapy for LCA. Therefore, we have ca...

Materials such as waste tire chips were widely used to improve the strength of soil. The objective of this study is to discuss the residual strength or steady-state behavior of sand-waste tire chip mixtures. A series of undrained monotonic triaxial compression tests were conducted on reconstituted saturated specimens of sand and sand-tire chip mixtures with variation in the tire-chip contents f...

the aim of this study is experimental assay of sensitivity and stability of a bimetallic silver/gold spr sensor chip. this chip utilizes the sensitivity of the silver and the stability of the gold. moreover, the silver layer (instead of usual cr or ti layer) was used as an adhesive intermediate layer between the gold layer and the glass substrate. the optimization of the gold/silver thickness u...

OBJECTIVES Hearing loss is the most common sensory disorder in humans and genetic causes are estimated to cause more than 50% of all incidents of congenital hearing loss. To develop an efficient method for a genetic diagnosis of hearing loss, we have developed and validated a genetic hearing loss DNA chip that allows the simultaneous analysis of 7 different mutations in the GJB2, SLC26A4, and t...

DNA chips are a promising fundamental technology for the post-genome research [1]. A large number of DNA oligonucleotide probes integrated in a small area of the chip surface facilitate speedy simultaneous detection of many target sequences. So, expression profile analysis of thousands of genes can be finished in a few days by using DNA chip technology although it takes years to finish the anal...

We describe, for the first time, the generation of a viral DNA chip for simultaneous expression measurements of nearly all known open reading frames (ORFs) in the largest member of the herpesvirus family, human cytomegalovirus (HCMV). In this study, an HCMV chip was fabricated and used to characterize the temporal class of viral gene expression. The viral chip is composed of microarrays of vira...

Nowadays, faults and failures are increasing especially in complex systems such as Network-on-Chip (NoC) based Systems-on-a-Chip due to the increasing susceptibility and decreasing feature sizes. On the other hand, fault-tolerant routing algorithms have an evident effect on tolerating permanent faults and improving the reliability of a Network-on-Chip based system. This paper presents reliabili...