the aim of this study was to determine the quality of cumulus-oocyte-complexes (cocs) and the effects of bovine serum albumin (bsa) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. cocs were collected from slaughterhouse buffalo ovaries by aspiration method. only normal grades cocs were matured for 48 hours in tcm-199 media. two groups were created: one for the ...

Abstract We describe a synthetic riboswitch element that implements regulatory principle which directly addresses an essential tRNA maturation step. Constructed using rational in silico design approach, this regulates RNase P-catalyzed 5?-processing by either sequestering or exposing the single-stranded 5?-leader region of precursor response to ligand. A single base pair defines potential both ...

Oocyte maturation depends on multiple variables. In fact, in dogs, the requirements for oocyte development in vitro are so complex that achievement of female gamete maturation has been viewed as a difficult problem. The conundrum, thus far unanswered, of achieving dog oocyte maturation in vitro, is nowadays the major puzzle of the in vitro program in the canine species. In in vitro studies of d...

The procedure for bovine in vitro fertilization (NF) involving the collection of ovaries at the slaughterhouse, maturation of oocytes in vitro (NM), capacitation and insemination in vitro and development to blastocysts in vitro is well established. Transportation of ovaries in physiological saline at 23 and 38°C afforded a large number of oocytes capable to develop to blastocysts after in vitro...

OBJECTIVE To examine whether in vitro differentiation of germ cells from men with maturation arrest is improved by augmenting FSH and T concentrations above the values effective in samples from men with normal spermatogenesis. DESIGN Prospective, controlled in vitro study. SETTING Private assisted reproduction centers and a university department. PATIENT(S) Men with meiotic or postmeiotic...

Background: Because of the problems of achieving to mature oocytes, abillity to deliver the oocytes to mature outside of ovaries was a big discovery that somewhat could reduce the risk of ART`s.Because of the low percentage of in vitro oocyte maturation, research for improving the IVM condition and increasing the oocyte maturation rate is to be continued. The aim of this research is the study o...

This paper presents the results of recovering horse oocytes by aspiration and maturation in vitro for 24, 30, 36 or 42 h. A total of 522 oocytes were recovered from 221 ovaries (2.4 per ovary) and 271 oocytes (51.9%) were selected for in vitro maturation (IVM). Oocytes were cultured in maturation medium (TCM 199 + estrus cow serum [ECS] + follicle-stimulating hormone [FSH] + 17 beta-estradiol +...

The physiology of oocyte in vitro maturation remains elusive. Generally, the oocytes have a very low maturation rate under in vitro conditions. In the current study, we found that melatonin promotes the maturation of oocytes in which mitochondria play a pivotal role. It was identified that; (1) mitochondria are the major sites for melatonin synthesis in oocytes and they synthesize large amounts...

Background: Physiological effects of obesity are increasingly common in women of reproductive age and are associated with infertility. Obese women have dysfunction in hypothalamic-pituitary-ovarian axis, menstrual cycle and are up to three times more likely to have decreased oligo-/anovulation. The purpose of present study was to evaluate the high fat diet on IVM and IVF outcomes in mouse. Mate...