Both influx and efflux transporters are thought to be involved in the intestinal absorption of fexofenadine. The present study examined the influx transporter-mediated intestinal absorption of fexofenadine in rats, focusing on the role of rat oatp3 (Oatp1a5). The intestinal permeability of fexofenadine was evaluated by means of the Ussing chamber method in the presence of a P-glycoprotein inhib...

Homologous recombinant tilapia growth hormone (rtGH) was tested for its effects on calcium metabolism in freshwater tilapia Oreochromis mossambicus . Fish were fed an optimal ration of 5% of their body mass per day. A positive correlation was found between the amount of food given and the branchial calcium influx. In male tilapia, the mean calcium influxes were 5.80 and 11.71 mmol h21 100 g21 w...

In this study, a 1-D transient state mechanistic model of cuttings transport with foam in inclined wells has been developed. The model is solved numerically to predict the optimum foam flow rate (liquid and gas rate) and rheological properties that would maximize cuttings transport efficiency in inclined wells. A detailed sensitivity analysis of the effect of gas and liquid flow rates, drilling...

Sodium and potassium ion contents and fluxes of isolated resting human peripheral polymorphonuclear leukocytes were measured. In cells kept at 37 degrees C, [Na]i was 25 mM and [K]i was 120 mM; both ions were completely exchangeable with extracellular isotopes. One-way Na and K fluxes, measured with 22Na and 42K, were all approximately 0.9 meq/liter cell water . min. Ouabain had no effect on Na...

Sodium and potassium ion contents and fluxes of isolated resting human peripheral polymorphonuclear leukocytes were measured . In cells kept at 37 °C, [Na]i was 25 mM and [K]i was 120 mM; both ions were completely exchangeable with extracellular isotopes . One-way Na and K fluxes, measured with 22Na and 42K, were all -0.9 meq/liter cell water -min . Ouabain had no effect on Na influx or K efflu...

Neuronal cells communicate with each other by producing electrical signals or action potentials (APs). Different ion channels, including Na+, K+ and Ca2+ channels, are involved in generation of AP. Once an AP is generated in the soma, it travels down entire the axon length toward its terminal in a self-generating fashion that ultimately conveys information between neurons in the neural circuit....

In the fight against tuberculosis, cell wall permeation of chemotherapeutic agents remains a critical but largely unsolved question. Here we review the major mechanisms of small molecule penetration into and efflux from Mycobacterium tuberculosis and other mycobacteria, and outline how these mechanisms may contribute to the development of phenotypic drug tolerance and induction of drug resistan...

Neutrophils loaded with the calcium indicator quin-2 and challenged with the ionophore ionomycin or the chemotactic peptide fMet-Leu-Phe were examined in the light of a theory that relates time-dependent changes in the fluorescence of the indicator to cytosolic calcium fluxes and levels. The cytosolic binding capacity was estimated from the theory to be 1.5 +/- 0.6 X 10(8) sites/cell (0.76 mM b...

Trypanosome lytic factor 1 (TLF1) is a subclass of human high-density lipoprotein that kills some African trypanosomes thereby protecting humans from infection. We have shown that TLF1 is a 500 kDa HDL complex composed of lipids and at least seven different proteins. Here we present evidence outlining a new paradigm for the mechanism of lysis; TLF1 forms cation-selective pores in membranes. We ...