A chitinase has been obtained in milligram amounts from wheat germ by affinity chromatography on chitin followed by chromatography on Sephadex G-50. The purified enzyme was free from wheat germ agglutinin and showed a single peak on sodium dodecyl sulfateacrylamide gel electrophoresis. Isoelectric focussing on acrylamide gel slabs gave rise, however, to several bands, with isoelectric points ra...

BACKGROUND Variations in sugar yield due to genotypic qualities of feedstock are largely undescribed for pilot-scale ethanol processing. Our objectives were to compare glucose and xylose yield (conversion and total sugar yield) from straw of five winter wheat cultivars at three enzyme loadings (2.5, 5 and 10 FPU g-1 dm pretreated straw) and to compare particle size distribution of cultivars aft...

Xylanase production from Bacillus pumilus VLK-1 in solid state fermentation was enhanced through optimization of the process using one variable approach. The enzyme production was highest (42324±786 IU/g) upon 96h of incubation at 37°C using wheat bran as a substrate moistened with mineral salt solution (pH 9.0) containing 0.1% folic acid, 0.2% riboflavin and 6.0% yeast extract in the ratio of ...

The DE values and digestible nutrients content of 6 diets were measured in 60-kg male growing pigs fed restricted amount of feed. Diets were prepared from 5 ingredients [wheat (Triticum aestivum), corn (Zea mays), barley (Hordeum vulgare), wheat bran, and soybean (Glycine max) meal; inclusion levels of ingredients were not correlated] with or without carbohydrose enzyme (Rovabio Excel AP; 3300 ...

Photosynthetic PGKinase (EC 2.7.2.3) is found in the stroma of chloroplasts, where it functions in the Calvin cycle, catalyzing the reversible, ATP-dependent conversion of 3-phosphoglycerate to 1,3-diphosphoglycerate. In wheat the enzyme is encoded by a single-copy, nuclear gene (Chao et al., 1989). We have shown that the expression of many of the Calvin cycle enzymes, including Fru-1,6bisphosp...

β-Amylase is used in the production of maltose syrup and preventing retrogradation of rice cake confectionery. However, until now the supply source for β-amylase has been limited to edible plants such as barley, wheat and soybean. Considering the current worldwide food crisis, it is the responsibility of enzyme producers to explore alternative sources for a stable and constant enzyme supply goi...

A cDNA that encodes a methyltransferase (MT) was cloned from a cold-acclimated wheat (Triticum aestivum) cDNA library. Molecular analysis indicated that the enzyme WPEAMT (wheat phosphoethanolamine [P-EA] MT) is a bipartite protein with two separate sets of S-adenosyl-L-Met-binding domains, one close to the N-terminal end and the second close to the C-terminal end. The recombinant protein was f...

Estrogen is metabolized along two competing pathways to form the 2-hydroxylated and the 16alpha-hydroxylated metabolites. Based on proposed differences in biological activities, the ratio of these metabolites, 2-hydroxyestrogen:16alpha-hydroxyestrone (2:16alpha-OHE1), has been used as a biomarker for breast cancer risk. Women with an elevated 2:16alpha-OHE1 ratio are hypothesized to be at a dec...

A fungal strain of Aspergillus oryzae was used for the production of alpha amylase by solid state fermentation from agro-industrial waste. Enzyme production was growth associated and maximum activity (8.23 U/ml) were obtained after 120h when incubated at 30°C on wheat bran with initial moisture content 60%; initial medium pH = 5 . Enzyme activity increased when the solid medium was supplemented...

This study presents production of α-amylase by Aspergillus oryzae in solid-state fermentation using 14 agro-industrial wastes as substrate. Enzyme production was growth associated and maximum titers (15095 U/gds) were obtained after 72 h when incubated at 30°C on wheat bran (initial moisture content, 60%; initial medium pH, 5). Enzyme titers increased significantly when the solid medium was sup...