A new type of holonomic omnidirectionul vehicle which consists of wheel mechanisms without free rollers but with conventional tires is presented. We first present a concept of the new driving wheel mechanism with a tire in the conventional style such QS a rubber tire or a pneumatic tire,"Offset Steered Driving Wheel". The configuration of the wheel has an offset distance between a wheel axle an...

DNA replication is an essential and conserved process in all domains of life and may serve as a target for the development of new antimicrobials. However, such developments are hindered by subtle mechanistic differences and limited understanding of DNA replication in pathogenic microorganisms. Clostridium difficile is the main cause of healthcare-associated diarrhoea and its DNA replication mac...

Ring-shaped sliding clamps and clamp loader ATPases are essential factors for rapid and accurate DNA replication. The clamp ring is once opened and resealed at the primer-template junctions by ATP-fueled clamp loader function. Processivity of DNA polymerase is conferred by attachment to the clamp loaded onto DNA. In eukarya and archaea, the hetero-pentemeric replication factor C (RFC) and the p...

The bacteriophage T4 DNA polymerase holoenzyme is composed of the core polymerase, gene product 43 (gp43), in association with the "sliding clamp" of the T4 system, gp45. Sliding clamps are the processivity factors of DNA replication systems. The T4 sliding clamp comes to encircle DNA via the "clamp loader" activity inherent in two other T4 proteins: 44 and 62. These proteins assemble into a pe...

The Escherichia coli beta dimer is a ring-shaped protein that encircles DNA and acts as a sliding clamp to tether the replicase, DNA polymerase III holoenzyme, to DNA. The gamma complex (gammadeltadelta'chipsi) clamp loader couples ATP to the opening and closing of beta in assembly of the ring onto DNA. These proteins are functionally and structurally conserved in all cells. The eukaryotic equi...

The static rigidity of a rotating spindle in the radial direction is investigated in this research. A magnetic loading device (magnet loader) has been developed for the measurement. The magnet loader, which has coils and iron cores, generates the electromagnetic force and attracts a dummy tool attached to the spindle. However, the eddy current is generated in the dummy tool with the spindle rot...

The sliding clamps of chromosomal replicases are acted upon by both the clamp loader and DNA polymerase. Several other proteins and polymerases also interact with the clamp. These proteins bind the clamp at the same spot and use it in sequential fashion. First the clamp loader must bind the clamp in order to load it onto DNA, but directly thereafter the clamp loader must clear away from the cla...

The replication clamp PCNA and its loader RFC (Replication Factor C) are central factors required for processive replication and coordinated DNA repair. Recently, several additional related clamp loaders have been identified. These alternative clamp loaders contain the small Rfc2-5 subunits of RFC, but replace the large Rfc1 subunit by a pathway-specific alternative large subunit, Rad24 for the...

We describe an efficient compiler and run-time system for NextGen, a compatible extension of the Java programming language supporting run-time generic types designed by Cartwright and Steele. The resulting system is comparable in performance with both standard Java and the GJ extension of Java, which does not support run-time generic types. Our implementation of NextGen consists of a compiler e...