Previous studies indicated a correlation between the formation of EDTA-resistant (irreversible) platelet-fibrinogen interactions and platelet cytoskeleton formation. The present study explored the direct association of membrane-bound fibrinogen with the Triton X-100 (Sigma Chemical Co, St Louis, MO) insoluble cytoskeleton of aspirin-treated, gel-filtered platelets, activated but not aggregated ...

BACKGROUND Blood samples for neonatal screening for inborn errors of metabolism are collected and shipped on standardized filter paper cards. Occasionally these samples are contaminated with EDTA, which is often used for anticoagulation. EDTA may interfere with newborn screening tests based on lanthanide fluorescence and thus lead to false-negative or false-positive results. METHODS We used t...

INTRODUCTION The aim of this study was to evaluate the effect of DJK-5, a newly developed cationic antimicrobial peptide, on oral multispecies and Enterococcus faecalis biofilms alone or combined with the endodontic chelating agent EDTA in vitro. METHODS Oral multispecies biofilms from 2 donors and E. faecalis VP3-181 biofilm were grown on collagen-coated hydroxyapatite disks. After incubatio...

BACKGROUND The aim was to evaluate the reproducibility of glomerular filtration rate (GFR) measured with iohexol and its response to food in a direct and independent comparison with Cr-51-ethylenediaminetetraacetic acid (EDTA), and examine the influence of two different whole body scaling parameters, body surface area (BSA) and extracellular fluid volume (ECV). METHODS Fasting and non-fasting...

Objective: This study aimed to evaluate whether platelet indices and leukocyte count predict the diagnosis of Ethylene Diamine Tetra Acetic Acid induced pseudothrombocytopenia (EDTA-PTCP) differ this entity from immune thrombocytopenia (ITP). Methods: One-hundred sixty-nine individuals (52 patients diagnosed with EDTA-PTCP, 52 newly ITP, 65 healthy individuals) were included. Demographic data c...

THE BASIS FOR EDTA-STIMULATION OF METHEMOGLOBIN REDUCTION IN HEMOLYSATES OF HUMAN ERYTHROCYTES Lucy Jean Sannes* and Donald E. Hultquist ** Department of Biological Chemistry The University of Michigan Ann Arbor, Michigan 48109 Received November lo,1979 Summary: We have studied the stimulation by EDTA of methemoglobin reduction in hemolysates of human erythrocytes. The EDTA effect has been show...

AIM The aim of this study was to evaluate the effect of dentin on the antimicrobial efficacy of 3% sodium hypochlorite, 2% chlorhexidine, 17% EDTA and 18% etidronic acid against C. albicans. METHODOLOGY Dentin powder was prepared from mandibular first premolar using electrical grinder and sterilized. 3% NaOCl, 2%CHX, 17% EDTA and 18% etidronic acid were tested against C. albicans in the prese...

Eggs from the sea urchin, Lytechinus pictus, were injected with either EGTA or EDTA, and were subsequently fertilized. EGTA prevented cortical vesicle discharge and formation of the fertilization membrane. EDTA had either no effect, or sometimes retarded the elevation of the fertilization membrane, or reduced the percentage of eggs with elevated membranes. Theoretical considerations lead to est...

The lysis of certain gram-negative bacteria by ethylenediaminetetraacetic acid (EDTA) and lysozyme was first reported by Repaske (7, 8). Lysis by EDTA alone was reported for Pseudomonas aeruginosa (3, 8), Vibrio succinogenes (9), and Alkaligenes faecalis (3). The mechanism for lysis by EDTA (2) in conjunction with lysozyme has been attributed to the interruption of the integrity of the cell sur...

would have been expressed in fewer words! Another gentleman with true military fortiter in re friendship, told me my pamphlet was tit to line trunks with, reminding one of what O'Connell said to a writer, *'1 saw an excellent thing in your last essay !" " What was it ? what was it ?" eagerly cried Scriblerus. "It was a mutton pie," replied O'Connell!. I have therefore good reason to remember Lo...