The aconitase AcnA from the phosphinothricin tripeptide producing strain Streptomyces viridochromogenes Tü494 is a bifunctional protein: under iron-sufficiency conditions AcnA functions as an enzyme of the tricarboxylic acid cycle, whereas under iron depletion it is a regulator of iron metabolism and oxidative stress response. As a member of the family of iron regulatory proteins (IRP), AcnA bi...

DNA methylation biomarkers for noninvasive diagnosis of colorectal cancer (CRC) and precursor lesions have been extensively studied. Different panels have been reported attempting to improve current protocols in clinical practice, although no definite biomarkers have been established. In the present study, we have examined patient biopsies starting from a comprehensive analysis of DNA methylati...

background and objectives: timely diagnosis of leptospirosis is essential for an effective treatment. large diversity of clinical symptoms has led leptospirosis diagnosis difficult. researchers have conducted many tests with wide-range of sensitivity and specificity to achieve novel diagnostic procedures which have higher sensitivity and specificity compared with previous tests and which are mo...

conclusions based on the findings, application of recombinant arcaga antigen for the detection of h. pylori infection is a simple, rapid, and brief non-invasive method. therefore, it can be suggested as an appropriate antigen for h. pylori detection kits. results we investigated caga protein, using the immunoblotting method in stool samples, which were already identified as positive by the appl...

background alcoholic hepatitis (ah) is an acute manifestation of alcoholic liver disease with high mortality rates. objectives our aim was to study the molecular mechanisms of ah. materials and methods the differentially expressed genes (degs) in liver between ah and control cases were identified by analyzing the gse28619 microarray data using t-test. the kyoto encyclopedia of genes and genomes...

an experiment was conducted to study wheat grain protein increasing through foliar application of nitrogen afterflowering. the rcbd design was analysed with three replications and four n-treatments as foliar application as(n0=0, n1=4kg.ha-1 urea, n2=8kg.ha-1 urea, n3=4kg.ha-1 ammonium sulfate). results showed that seed proteincontent increased significantly by foliar application of nitrogen. da...

objectives the overall goal of this study was to carry out a set of comparative analyses of arsr gene in plasmid r773 and bacterial chromosome from escherichia coli bl-21(de3). methods pdb and ncbi databases and chimera, mega4, clc main workbench software and 3d-jigsaw and embl-ebi servers were applied to perform this study. by using these software and servers, multiple analyses including deter...

Adaptabilities of mammalian cells in physiological confinements of the vasculature and tissue-matrices remain unaddressed. As the adaptation to coupled chemo-mechanical stimuli becomes a pivotal factor for cell survival, we investigate here the prospect of confinement induced alterations in the stress adaptive behavior of mammalian cell lines. To comprehend the physical dynamics of cells during...

To elucidate the pathways by which nitric oxide (NO) influences macrophage iron metabolism, the uptake, release, and intracellular distribution of iron in the murine macrophage cell line J774 has been investigated, together with transferrin receptor (TfR) expression and iron-regulatory protein (IRP1 and IRP2) activity. Stimulation of macrophages with interferon-gamma (IFN-gamma) and/or lipopoly...

BACKGROUND High-resolution manometry (HRM) has improved the accuracy of manometry in detecting achalasia and has helped distinguish between clinically relevant subtypes. This study investigated whether HRM metrics correlate with the achalasia symptoms and symptomatic outcomes of peroral esophageal myotomy (POEM). METHODS Of the 30 patients who were enrolled, 25 were treated with POEM, 12 of w...