Anacardic acid C15:3 and cardol C15:3 sigmoidally suppressed superoxide anion (O2-) generation using xanthine oxidase. To study this suppression activity, anacardic acids, cardanols and cardols having different numbers of double bonds in alk(en)yl chains were prepared. The O2- scavenging activity and H2O2 formation from O2- using PMS-NADH were examined. Anacardic acids and cardols indicated sig...

The relationship of folic acid (PGA) to certain enzyme systems has recently been the subject of investigation by workers in several laboratories. In 1948 Kalckar and Klenow (l-3) demonstrated the inhibitory effect of folic acid upon milk xanthopterin oxidase in vitro. Subsequently Keith et al. (4), studying the effect of dietary PGA upon enzymes, observed that activity of xanthine oxidase in ch...

The relationship of folic acid (PGA) to certain enzyme systems has recently been the subject of investigation by workers in several laboratories. In 1948 Kalckar and Klenow (l-3) demonstrated the inhibitory effect of folic acid upon milk xanthopterin oxidase in vitro. Subsequently Keith et al. (4), studying the effect of dietary PGA upon enzymes, observed that activity of xanthine oxidase in ch...

The endogenous reactive oxygen species ("ROS") formation is associated with many pathologic states such as inflammatory diseases, neurodegenerative diseases, brain and heart ischemic injuries, cancer, and aging. The purpose of this study was to investigate the endogenous sources for "ROS" formation in intact isolated rat hepatocytes, in particular, peroxisomal oxidases, monoamine oxidase, xanth...

The xanthine oxidase activity of mouse regenerating liver has been shown to be elevated during the period of rapid liver growth and proliferation. This increase is evident when the enzyme activity is expressed per unit wet tissue weight, per unit nitrogen, or per cell. The adrenal cortex probably plays only a minor role in implementing this phenomenon. Further augmentation of the xanthine oxida...

An optimum way of immobilizing xanthine oxidase on graphite was found where a redox transformation of the enzyme was observed. The nature of the redox maxima was hypothesized on the basis of the dependence of the half-wave potential (Ep/2) on the pH of the solution. The enzymatic activity of xanthine oxidase adsorbed on two kinds of soot was studied by the oxidation of xanthine. The kinetic and...