A 12 kDa cysteine-rich protein is secreted by Fusarium oxysporum f. sp. lycopersici during colonization of tomato xylem vessels. Peptide sequences obtained with mass spectrometry allowed identification of the coding sequence. The gene encodes a 32 kDa protein, designated Six1 for secreted in xylem 1. The central part of Six1 corresponds to the 12 kDa protein found in xylem sap of infected plant...

The effects of placing solid implants containing Fe sulfate in branches of Fe-deficient pear and peach trees on the composition of the xylem sap have been studied. Iron sulfate implants are commercially used in northeastern Spain to control iron chlorosis in fruit trees. Implants increased Fe concentrations and decreased organic acid concentrations in the xylem sap, whereas xylem sap pH was onl...

Freezing point depression in xylem sap of mangroves was found to range from 0.05 to 0.5 degrees , in desert plants from 0.01 to 0.16 degrees . In crush juices from leaves of Batis and Salicornia, 90% or more of the freezing point depression was made up of sodium and chlorine ions; in mangroves they constituted 50 to 70%, the rest probably being organic solutes. Plants growing in seawater have -...

Abstract In higher plants, the xylem, which is crucial for water and nutrient transport, also contains a diverse proteome. The composition of xylem sap proteome responds dynamically to changes in environmental conditions. Important functions are maintenance structural integrity defense against invading pathogens. Knowledge on signals that trigger proteomic scarce. Better understanding these pro...

Analyses of host plant resistance / susceptibility mechanisms to Xylella fastidiosa (Xf) infection are critical for understanding host-pathogen interactions. Proteomic analyses of stem tissue and xylem sap samples were initiated to complement genomic approaches employed in elucidating Pierce’s disease resistance mechanisms. Samples from one highly resistant and two susceptible grape genotypes w...

A modified version of a method that uses positive air pressures to determine the complete cavitation response of a single axis is presented. Application of the method to Betula occidentalis Hook, gave a cavitation response indistinguishable from that obtained by dehydration, thus verifying the technique and providing additional evidence that cavitation under tension occurs by air entry through ...

Land plants evolved xylem vessels to conduct water and nutrients, and to support the plant. Microarray analysis with a newly established Arabidopsis in vitro xylem vessel element formation system and promoter analysis revealed the possible involvement of some plant-specific NAC-domain transcription factors in xylem formation. VASCULAR-RELATED NAC-DOMAIN6 (VND6) and VND7 can induce transdifferen...

The ability of DNA to adopt non-canonical structures can affect transcription and has broad implications for genome functioning. We have recently reported that guanine-rich (G-rich) homopurine-homopyrimidine sequences cause significant blockage of transcription in vitro in a strictly orientation-dependent manner: when the G-rich strand serves as the non-template strand [Belotserkovskii et al. (...