The value of combining informations obtained from different methods, for gene function predictions, has been illustrated by several studies [1, 2, 3, 4]. We propose a new scoring framework, called Adaptive Score (AdS), for predicting the function of a few unclassified Yeast genes. We mainly focus on phenotypic profiles [5], microarray gene expression (All Yeast data [6]), KEGG pathway database ...

We describe a general method for plasmid assembly that uses yeast and extends beyond yeast-specific research applications. This technology exploits the homologous recombination, double-stranded break repair pathway in Saccharomyces cerevisiae to join DNA fragments. Synthetic, double-stranded "recombination linkers" were used to "subclone" a DNA fragment into a plasmid with > 80% efficiency. Qua...

Construction of synthetic genetic networks requires the assembly of DNA fragments encoding functional biological parts in a defined order. Yet this may become a time-consuming procedure. To address this technical bottleneck, we have created a series of Gateway shuttle vectors and an integration vector, which facilitate the assembly of artificial genes and their expression in the budding yeast S...

automatic analysis of human facial expressions is one of the challenging problems in machine vision systems. it has many applications in human-computer interactions such as, social signal processing, social robots, deceit detection, interactive video and behavior monitoring. in this paper, we develop a new method for automatic facial expression recognition based on facial muscle anatomy and hum...

background and objectives: hbha and mtb32c have been isolated from culture supernatants of mycobacterium tu- berculosis (m. tuberculosis) and mycobacterium bovis (m. bovis) and their immunogenicity previously studies have been confirmed. in this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (mt- b32c-hbha), in producing new chimeric protein under t...

conclusions: our data showed that the recombinant mature lysostaphin protein produced by pet32a vector in e. coli system was very efficient. results: pcr and sequencing results confirmed the successful cloning of the target gene into the vector. the expression of protein was induced by iptg and high concentration of the recombinant protein was obtained via the purification process by affinity-c...

Bleomycin is a well-established anti-tumor drug. Its major untoward effect, pulmonary toxicity, has limited its usage. In this study, we used a DNA repair protein, yeast apurinic/apyrimidinic endonuclease (APN1) to reduce the toxicity of bleomycin on lung cells. A549 cells, an alveolar epithelial cell line, were transduced by MIEG3 retroviral vector encoding both enhanced green fluorescent prot...

objective: induced pluripotent stem cells are generated from somatic cells by direct reprogramming. these reprogrammed pluripotent cells have different applications in biomedical fields such as regenerative medicine. although viral vectors are widely used for efficient reprogramming, they have limited applications in the clinic due to the risk for immunogenicity and insertional mutagenesis. acc...

for high-throughput production of recombinant protein in escherichia coli ( e. coli ), besides important parameters such as efficient vector with strong promoter and compatible host, other important issues including codon usage, rare codons, and gc content specially at n-terminal region should be considered. in the current study, the effect of decreasing the percentage of gc nucleotides and opt...

objective: development of high producing mammalian cell lines is a major bottleneck in manufacturing of recombinant therapeutic proteins. this study examines the effect of using the matrix attachment region from the human interferon beta gene in combination with promoter activation strategy with e1a 13s protein on human tissue plasminogen activator (t-pa) expression in chinese hamster ovary (ch...