In food/feed control, mycotoxin analysis is often still performed "one analyte at a time". Here a method is presented which aims at making mycotoxin analysis environmentally friendlier through replacing acetonitrile by ethyl acetate and reducing chemical waste production by analyzing four mycotoxins together, forgoing sample extract clean-up, and minimizing solvent consumption. For this, 2 g of...

Grape pomace (pulp and skins) was investigated as a new biosorbent for removing mycotoxins from liquid media. In vitro adsorption experiments showed that the pomace obtained from Primitivo grapes is able to sequester rapidly and simultaneously different mycotoxins. Aflatoxin B1 (AFB1) was the most adsorbed mycotoxin followed by zearalenone (ZEA), ochratoxin A (OTA), and fumonisin B1 (FB1), wher...

In this study, a rapid multi-mycotoxin approach was developed for biomonitoring and quantification of 27 important mycotoxins and mycotoxin metabolites in human blood samples. HPLC-MS/MS detection was used for the analysis of dried serum spots (DSS) and dried blood spots (DBS). Detection of aflatoxins (AFB1, AFB2, AFG1, AFG2, AFM1), trichothecenes (deoxynivalenol, DON; DON-3-glucoronic acid, DO...

Breast milk substitutes, e.g. infant formulae, are commonly introduced to the diet within first months of life. As infants’ detoxifying capability is not fully developed, it vital importance minimize their exposure food contaminants. Here, we present a comprehensive multi-mycotoxin assessment in food. Samples from Austrian and Czech market (n = 59) were screened for 46 mycotoxins key metabolite...

Previous studies using zearalenone (ZEN) and fumonisins (FB) revealed alpha-zearalanol (?-ZOL) FB1 in the liver of turkeys chickens with no sign toxicity. The aim present study was to determine whether contamination persists after distribution a mycotoxin-free diet for several days. Turkeys broilers were fed 14 days containing respectively, 7.5 0.6 mg/kg FB ZEN, then 0, 2 or 4 diet. total ?-ZOL...

Mycotoxins are secondary metabolites of fungi that grow on a variety of grains, feedstuffs and animal feed. Feed containing moderate to high levels of mycotoxins cause clinical symptoms in most animals, ranging from organ damage to acute mortality. Consumption of low levels of contaminated feed may result in subclinical problems such as immunosuppression. More than 500 mycotoxins have been iden...

Rice bran, a by-product of the rice milling process, has emerged as functional food and being used in formulation healthy drinks. However, bran is often contaminated with numerous mycotoxins. In this study, method to simultaneous detection aflatoxins (AFB1, AFB2, AFG1, AFG2), ochratoxin A (OTA), deoxynivalenol (DON), fumonisins (FB1 FB2), sterigmatocystin (STG), T-2 toxin, HT-2 diacetoxyscirpen...

The fungi of the genus Fusarium cause Fusarium head blight (FHB), a devastating disease that reduces grain yield and quality. They also produce mycotoxins which may pose a serious threat to human and animal health. This study investigated the effects of NPK fertilisation, foliar application of Cu, Zn, and Mn, applied separately and in combination, and of the Nano-Gro® organic growth stimulator ...