Effects of temperature and hydrostatic pressure were measured on cytosolic malate dehydrogenases (cMDHs) from muscle tissue of a variety of shallowand deep-living benthic marine invertebrates, including seven species endemic to the deep-sea hydrothermal vents. The apparent Michaelis-Menten constant (Km) of coenzyme (nicotinamide adenine dinucleotide, NADH), used to index temperature and pressur...

Discrete and continuous measurement of indoor radon concentrations, air flow rates, and pressure differentials for passive and active operational configurations of a passive radon control system were performed at the Biotechnology Research and Development Facility of the University of Florida, in Alachua County, Florida. The system was installed during construction and incorporated an extended ...

Since the discovery of hydrothermal vents 40-years ago, long-term time-series have focused on mid-ocean ridge systems. Based on these studies, hydrothermal vents are widely considered to be dynamic, ephemeral habitats. Under this premise, national, and international regulatory bodies are currently planning for the commercial mining of polymetallic sulfide deposits from hydrothermal vents. Howev...

Dharma/Bozozok (Dha/Boz) is a homeodomain protein containing an Engrailed homology (Eh) 1 repressor motif. It is important in zebrafish dorsal organizer formation. Dha/Boz interacted with a co-repressor Groucho through the Eh1 motif. Expression of a Dha/Boz fused to the transcriptional activator VP16 repressed dorsal axis formation and the expression of organizer genes but led to the dorsal exp...

Hydrothermal vent fluids are highly enriched in iron (Fe) compared to ambient seawater, and organic ligands may play a role in facilitating the transport of some hydrothermal Fe into the open ocean. This is important since Fe is a limiting micronutrient for primary production in large parts of the world’s surface ocean. We have investigated the concentration and speciation of Fe in several vent...

Vent vacuum relief valves (VRVs) are used to limit the negative pressure at the ventricular vent catheter tip as well as prevent reversal of blood flow and prevention of air embolism. The purpose of this study was to evaluate the performance of three commercially available ventricular vent valves. The negative pressure at which the vent valve opened was measured at the valve inlet using high-fi...

Reduced inorganic compounds of geothermal-origin hydrogen sulfide (H2S), iron (Fe[II]), and methane (CH4) were common but not ubiquitous components of hydrothermal vent fluids of Yellowstone Lake at concentrations capable of supporting chemolithoautotrophic (geochemical-oxidizing, carbon dioxide (CO2)-fixing) bacterial growth. Closely linked to the presence of reduced geochemicals was abundance...