The chemokine domain of fractalkine (FKN-CD) binds to the classical RGD-binding site of αvβ3 and that the resulting ternary complex formation (integrin-FKN-CX3CR1) is critical for CX3CR1 signaling and FKN-induced integrin activation. However, only certain cell types express CX3CR1. Here we studied if FKN-CD can activate integrins in the absence of CX3CR1. We describe that WT FKN-CD activated re...

PET imaging of integrin αvβ3 expression has been studied intensely by the academia and recently also by the industry. Imaging of integrin αvβ3 expression is of great potential value, as the integrin αvβ3 is a key player in tumor metastasis and angiogenesis. Therefore PET imaging of this target might be a suitable in-vivo biomarker of angiogenesis and metastatic potential of tumors. In this manu...

BACKGROUND Matrix metalloproteinase-2 (MMP-2) is a key regulator in the migration of tumor cells. αvβ3 integrin has been reported to play a critical role in cell adhesion and regulate the migration of tumor cells by promoting MMP-2 activation. However, little is known about the effects of MMP-2 on αvβ3 integrin activity and αvβ3 integrin-mediated adhesion and migration of tumor cells. METHODO...

introduction: the implantation is a complex procedure that involves many molecules. one of these molecules is integrin specially &alphav;&beta3; which serves as receptor for components of extra cellular matrix to act as bridging molecules between the blastocyst and the endometrial surface during the implantation process. by blocking &alphav;&beta3; interactions, the implantation can be impaired...

Neocortex expansion during evolution is associated with the enlargement of the embryonic subventricular zone, which reflects an increased self-renewal and proliferation of basal progenitors. In contrast to human, the vast majority of mouse basal progenitors lack self-renewal capacity, possibly due to lack of a basal process contacting the basal lamina and downregulation of cell-autonomous produ...

Experimental data in this study demonstrate that integrin αvβ3 is fundamentally involved in the maturation of blood vessels during embryonic neovascularization (vasculogenesis). Integrin αvβ3 was specifically expressed on the surface of angioblasts during vessel development in quail embryos and vitronectin, a ligand for αvβ3, localized to the basal surface of these cells. More importantly, micr...

The cell adhesion molecule integrin αvβ3 is an important player in the process of tumor angiogenesis and metastasis. Antibodies, peptides, peptidomimetics, and small molecule antagonists against integrin αvβ3 have been shown to induce endothelial apoptosis, to inhibit tumor angiogenesis, and to increase endothelial permeability. The ability to quantitatively image integrin αvβ3 expression in vi...

Integrin αvβ3 expression is altered in various diseases and has been proposed as a drug target. Here we use a rational design approach to develop a therapeutic protein, which we call ProAgio, that binds to integrin αvβ3 outside the classical ligand-binding site. We show ProAgio induces apoptosis of integrin αvβ3-expressing cells by recruiting and activating caspase 8 to the cytoplasmic domain o...

factor responses may be either pathways shared by many Maximilian Schneller, Kristiina Vuori and integrins, or they may be specific for a few integrins. In Erkki Ruoslahti1 some cases, a signal appears to be specific for one La Jolla Cancer Research Center, The Burnham Institute, particular integrin. Activation of the focal adhesion kinase 10901 North Torrey Pines Road, La Jolla, CA 92037, (FAK...

99mTc-3P-RGD2 and SPECT/CT were valuable tools for selecting patient likely benefit from integrin αvβ3 blocking therapy. To evaluate the feasibility of 99mTc-3P-RGD2 imaging to detect head and neck squamous cell carcinoma, 99mTc-3P-RGD2 was prepared and the relationship between its accumulation and integrin αvβ3 expression in nude mice bearing HEP-2 or CNE-1 carcinoma xenograft were analyzed. T...