β-Sheet-rich aggregates of α-synuclein (αSyn) are the hallmark neuropathology of Parkinson's disease and related synucleinopathies, whereas the principal native structure of αSyn in healthy cells--unfolded monomer or α-helically folded oligomer--is under debate. Our recent crosslinking analysis of αSyn in intact cells showed that a large portion of endogenous αSyn can be trapped as oligomers, m...

Thermostable β-galactosidases from thermophilic bacteria have attracted increasing interest to various advantages in industrial and biotechnological applications. In this study, a highly thermally stable β-galactosidase produced by Anoxybacillus sp. AH1was purified characterized. The highest enzyme production was achieved after the bacterium incubated for 24 hours. precipitation with ammonium s...

The open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. One possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. Themain purpose of this study was to compare the survival rates of in vitro produced b...

The present study aimed to investigate the effect of N-Ethylmaleimide (NEM) on evaluation disulfide formation in oxi dized myofibrillar proteins during sample preparation non-reducing SDS-PAGE procedure. For this purpose, extracted were oxidized firstly via a Fenton oxidation reaction, and non-oxidized used as control. Before running SDS-PAGE, preparation, these prepared according three dif fer...

OBJECTIVE Mesenchymal stem cells (MSCs) can differentiate into various cell types. Since cysteine has structural similarities to neuronal inducers β-mercaptoethanol and glutathione, we examined its effect on neural induction of rat bone marrow MSCs. MATERIALS AND METHODS In this experimental study, cells were treated in a medium containing 1mM cysteine for 24 hours prior to treatment with neu...