Background: Trinitrotoluene (TNT) is one of the most well-known and oldest explosive agents. In the recent decade, bioenvironmental, biochemical, and biological effects of TNT exposure have been more in the spotlight. In this study, we aimed to evaluate spirometric parameters in workers of a TNT factory exposed to TNT and other related fumes and dusts compared with the unexposed controls. Metho...

Trees belonging to the genus Populus are often used for phytoremediation due to their deep root formation, fast growth and high transpiration rates. Here, we study the capacity of transgenic hybrid aspen (Populus tremula x tremuloides var. Etropole) which expresses the bacterial nitroreductase gene, pnrA, to tolerate and take-up greater amounts of the toxic and recalcitrant explosive, 2,4,6-tri...

BACKGROUND A consensus document developed by a joint committee of the European Society of Cardiology and the American College of Cardiology redefines myocardial infarction (MI) using an increase of troponin I or T as compared to a reference control population (i.e., troponin T (TnT) of 0.01 microg/l). A clinical problem arises when an arbitrary cut-off point is selected for determination of MI ...

Troponin T (TnT) is an essential protein in the Ca2+ regulatory system of striated of muscle. Three fiber type-specific TnT genes have evolved in higher vertebrates to encode cardiac, slow and fast skeletal muscle TnT isoforms. To understand the functional significance of TnT isoforms, we studied the effects of acidosis on the contractility of transgenic mouse cardiac muscle that expresses fast...

Three fiber type-specific genes have evolved in vertebrates to encode cardiac, slow and fast skeletal muscle troponin Ts (TnT). From the transcript of each gene, alternative RNA splicing produces multiple TnT isoforms with structural differences in three variable regions of the polypeptide chain. The expression of TnT isoform is regulated during heart and muscle development and adaptation. Musc...

A field demonstration and longevity assessment for long-term monitoring of the explosive 2,4,6-trinitrotoluene (TNT) in a marine environment using an anti-TNT microfluidic immunosensor is described. The TNT immunosensor is comprised of a microfluidic device with 39 parallel microchannels (2.5 cm × 250 µm × 500 µm, L × W × D) fabricated in poly(methylmethacrylate) (PMMA), then chemically functio...

Troponin T (TnT), a thin filament myofibrillar protein, is essential for the Ca2+ regulation of striated muscle contraction in vertebrates, both in vivo and in vitro. To understand the role of TnT in this process, its interaction with two other troponin components, troponin I (TnI) and troponin C (TnC) was examined by using the yeast two hybrid system, which is a genetic approach to detect prot...

Nitroaromatic compounds are xenobiotics that have found multiple applications in the synthesis of foams, pharmaceuticals, pesticides, and explosives. These compounds are toxic and recalcitrant and are degraded relatively slowly in the environment by microorganisms. 2,4,6-Trinitrotoluene (TNT) is the most widely used nitroaromatic compound. Certain strains of Pseudomonas and fungi can use TNT as...