Fluorescence Recovery After Photobleaching (FRAP) has been a versatile tool to study transport and reaction kinetics in live cells. Since the fluorescence data generated by fluorescence microscopy are in a relative scale, a wide variety of scalings and normalizations are used in quantitative FRAP analysis. Scaling and normalization are often required to account for inherent properties of diffus...

The total phenolic content and related total antioxidant capacity of 70 medicinal plant infusions was analyzed. Infusions were prepared in common way in which teas are prepared for human consumption. The total phenolics were measured by Folin–Ciocalteau assay. The total antioxidant capacity was estimated by Ferric Reducing/Antioxidant Power (FRAP) assay. To make practical comparison of relative...

FRAP (fluoride-resistant acid phosphatase)-reactivity in the substantia gelatinosa of the mouse spinal trigeminal nucleus caudalis (STNC) was examined by light and electron microscopy. Degenerated figures of terminals caused by capsaicin were compared with the FRAP-positive terminals. Scalloped (fan-like) or indented, sinuous, slender, and cap-like figures with closely packed agranular synaptic...

Susceptibility to mouse plasmacytomagenesis is a complex genetic trait controlled by several Pctr loci (Pctr1, Pctr2, etc). Congenic strain analysis narrowed the genetic interval surrounding the Pctr2 locus, and genes identified in the interval were sequenced from susceptible BALB/c and resistant DBA/2 mice. Frap (FKBP12 rapamycin-associated protein, mTOR, RAFT) was the only gene differing in a...

Objectives. The total antioxidant capacity (TAC) of a sample can be measured with a ferric reducing antioxidant power (FRAP) assay. There are commercially available kits for FRAP assays, however they are more expensive than in-house kits. We aimed to evaluate a FRAP direct measurement method under our laboratory conditions using a microplate reader and establish reference values to use in futur...

3 Parameter Estimation 7 3.1 Model of FRAP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 3.2 FRAP simulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 3.3 FRAP experiments and Latrunculin A . . . . . . . . . . . . . . . . . . . . . . . . . . 8 3.4 Boff and Soff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery after Photobleaching (FRAP) are probably the most widely used methods to determine diffusion coefficients in biological samples. However, it has already been noted in the past that the two methods do not necessarily provide the same results. This has typically been explained by the different spatial range over which the two m...

Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological research. The method was developed in the 1970s, when its biological application mainly focused on the mobility of fluorescently labelled constituents of the cell membrane. The development of confocal scanning microscopy in the 1980s facilitated accur...

Seasonal discrepancy in the antioxidant potential from bark of Nothapodytes nimmoniana was evaluated using two different methods (DPPH and FRAP) and were related to total phenolic content as well as flavonoid content. Higher yield of phenolic and flavonoid were observed during summer (May) similarly, the antioxidant activities (DPPH & FRAP) also were high. The antioxidant activities were strong...