We used high-precision density and ultrasonic velocity measurements to characterize the native (N), molten globule (MG), and unfolded (U) conformations of apomyoglobin. The molten globule states that were studied in this work include the MG(pH4)(NaCl) state observed at pH 4 and 20 mM NaCl, the MG(pH4)(NaTCA) state observed at pH 4 and 20 mM sodium trichloracetate (NaTCA), the MG(pH2)(NaCl) stat...

1. ac-N-Methyl-oc-N-toluene-p-sulphonyl-L-lysine ,B-naphthyl ester (MTLNE) was synthesized as its hydrobromide and shown to be slowly hydrolysed by bovine pancreatic trypsin. The acylation step, however, is so much faster than deacylation of the acyl-enzyme that spectrophotometric measurement of the 'burst' of ,B-naphthol provides a convenient method for determining the absolute molarity of try...

We have carried out a computational structure-based design of new potent pyrrolidine carboxamide (PCAMs) inhibitors of enoyl-acyl carrier protein reductase (InhA) of Mycobacterium tuberculosis (MTb). Three-dimensional (3D) models of InhA-PCAMx complexes were prepared by in situ modification of the crystal structure of InhA-PCAM1 (Protein Data Bank (PDB) entry code: 4U0J), the reference compound...

Aims: Polyketide synthase 13 (Pks13) is an essential enzyme in the synthesis of mycolic acids biosynthesis pathway Mycobacterium tuberculosis (Mtb). Therefore, Pks13 a promising drug target for treatment. Here we report silico design and evaluation novel inhibitors made benzofuran derivatives with favorable predicted pharmacokinetic profiles.
 Methodology: A 3D model Pks13-TAMx complexes w...

In petunia, loci PH1 to PH7 are involved in an unknown vacuolar acidification pathway since disruption of these loci results in an increased vacuolar pH and bluish flowers. PH4 encodes a MYB protein, which regulates, together with regulators of the anthocyanin biosynthesis pathway (AN1, AN2 and AN11), the transcription of (unknown) structural genes involved in vacuolar acidification. Here we sh...

The negligible double kinetic deuterium isotope effect (k(HH)/k(DD)= 1.05) in the reaction where [2,3,4,5-Ph4(eta5-C4COH)Ru(CO)2H (2) transfers a hydride and a proton to N-phenyl-[1-(4-methoxyphenyl)ethylidene]amine (4) indicates that no bond to hydrogen is broken or formed in the rate-determining step.

Antibodies against enterocin A were obtained by immunization of rabbits with synthetic peptides PH4 and PH5 designed, respectively, on the N- and C-terminal amino acid sequences of enterocin A and conjugated to the carrier protein KLH. Anti-PH4-KLH antibodies not only recognized enterocin A but also pediocin PA-1, enterocin P, and sakacin A, three bacteriocins which share the N-terminal class I...

The present study aimed to evaluate the stability of active pharmaceutical ingredients (APIs) from different pharmacological classes in a compounded oral suspending vehicle. Oral suspensions amoxicillin trihydrate (50 mg/mL), clozapine (25 indomethacin (5.0 levodopa/carbidopa (10.0/2.5 levothyroxine sodium (T4, 25 µg/mL), lomustine (4.0 and 10.0 methyldopa mg/mL) procarbazine (10.0 were formula...