Objective: a1-Adrenergic receptors (ARs) are known mediators of a positive inotropy in the heart, which may play even more important roles in heart disease. Due to a lack of sufficiently selective ligands, the contribution of each of the three a1-AR subtypes (a1A, a1B and a1D) to cardiac function is not clearly defined. In this study, we used a systemically expressing mouse model that overexpre...

[1] This study examines the effects of future anthropogenic emissions on climate, and the resulting feedback to natural emissions and air quality. Speciated sectorand region-specific 2030 emission factors were developed to produce gas and particle emission inventories that followed Special Report on Emission Scenarios (SRES) A1B and B1 emission trajectories. Current and future climate model sim...

We have shown previously that the Ca channel b3 subunit is capable of modulating tonic G-protein inhibition of a1A and a1B Ca channels expressed in oocytes. Here we determine the modulatory effect of the Ca channel b3 subunit on M2 muscarinic receptor-activated G-protein inhibition and whether the b3 subunit modulates the G-protein sensitivity of a1A and a1B currents equivalently. To compare th...

Despite the high level of symmetry between PsaA and PsaB polypeptides in Photosystem I, some amino acids pairs are strikingly different, such as PsaA-Gly693 PsaB-Trp673, which located near a cluster 11 water molecules A1A A1B quinones FX iron-sulfur cluster. In this work, we changed PsaB-Trp673 to PsaB-Phe673 Synechocystis sp. PCC 6803. The variant contains ~ 85% wild-type (WT) levels I but is ...

To investigate a1B-adrenoceptor function, we developed a phosphorothioate antisense oligodeoxynucleotide (AO) to inhibit the expression of the a1B-adrenoceptor subtype in DDT1 MF2 cells. We measured the cellular uptake of the AO and its effect on a1B-adrenoceptor mRNA expression, protein density, and coupling to phospholipase C. Cells treated with either a control oligodeoxynucleotide (CO) or m...

Kirsten R. McDonald, Neva J. Fudge, Janine P. Woodrow, James K. Friel, Ana O. Hoff, Robert F. Gagel, and Christopher S. Kovacs Faculty of Medicine Endocrinology, Memorial University of Newfoundland, St. John’s, Newfoundland A1B 3V6; Department of Biochemistry and Pediatrics, Memorial University of Newfoundland, St. John’s, Newfoundland A1B 3X9, Canada; and University of Texas MD Anderson Cancer...

Selective inactivation of a1B-adrenoceptor (AR) by the sitedirected alkylating agent chlorethylclonidine (CEC) has been used as one of major pharmacological criteria to subclassify a1-AR; however, the mechanism for the differential CEC sensitivity of the two subtypes is uncertain, and the extent of CEC inactivation varies depending on the treatment employed. In this study, we examined the corre...