This study suggests one mechanism by which alveolar macrophages accumulate in the lung in pulmonary emphysema: elastin fragments generated at the diseased sites are potent chemoattractants for monocytes, the precursors of the macrophages. The most chemotactic elastin fragments have a molecular weight between 10,000 and 50,000 and are active at concentrations as low as 3 nanograms per milliliter...

(ii) Samples required: (a) The mitomycin used in making the batch: Five packages, each containing approximately 100 milligrams. (b) The batch: (1) For all tests except sterility: A minimum of 25 immediate containers. (2) For sterility testing: 20 immediate containers, collected at regular intervals throughout each filling operation. (b) Tests and methods of assay—(1) Potency. Proceed as directe...

Inhibition of the proliferation of Ehrlichia risticii cultured in murine macrophage P388D1 cells by eight antibiotics was evaluated by indirect fluorescent-antibody staining with an antiserum specific to E. risticii. There was a negative correlation between the percentage of infected cells and the log10 of the concentrations of all antibiotics examined. The ranks of the antibiotics in the order...

The inositol phospholipids, phosphatidylinositol monophosphate (PIP) and phosphatidylinositol bisphosphate (PIP(2)), have been shown to increase the vanadate-sensitive ATPase activity of plant plasma membranes (AR Memon, Q Chen, WF Boss [1989] Biochem Biophys Res Commun 162: 1295-1301). In this paper, we show the effect of various concentrations of phosphatidyinositol, PIP, and PIP(2) on the pl...

°C degrees Celsius 95% CI 95% confidence interval µg microgram ACTH adenocorticotropic hormone ALP alkaline phosphatase ALT alanine aminotransferase AST aspartate aminotransferase CETP cholesteryl ester transfer protein dL deciliter EDTA ethylenediaminetetraacetic acid GGT gamma-glutamyl transferase HDL high density lipoprotein IDL intermediate density lipoprotein L liter LCAT lecithin-choleste...

Thrombokinase has been isolated from bovine plasma by a procedure which begins with the highly purified product of a previously described method, chromatographs it on DEAE-cellulose, and then fractionates it by continuous flow electrophoresis, yielding 0.2 mg per liter of oxalated plasma. The electrophoretic fraction has shown a single boundary in the ultracentrifuge; and its esterase activity ...

The molecular nutritional requirements exhibited by mammalian cells can vary with the following conditions: whether the cells are plated singly or in massive inoculum; whether a clonal strain or a mixture of different genotypes is employed; whether the macromolecular fraction of serum used as a supplement was previously exposed to prolonged dialysis or other procedures which may release bound m...

This study was designed to determine the potential of IGF-1 as a neuronal rescue agent after cerebral ischemia. Unanesthetized late gestation fetal sheep were subjected to 30-min cerebral ischemia by inflation of carotid artery occluder cuffs. 2 h later either 0.1 microgram rhIGF-1, 1 microgram rhIGF-1, 10 micrograms rhIGF-1, or vehicle was infused into a lateral cerebral ventricle over 1 h. Hi...

The inductively coupled plasma is a promising excitation source for the simultaneous multielement determination of trace (nanogram per milliliter) elements in biological fluids. As little as 25 tl of sample fluid (whole blood, serum, or plasma) has been used, with essentially no sample preparation. This technique offers significant advantages over other methods requiring considerable sample han...