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DNA strand displacement is a process by which duplex invaded single-stranded nucleic acid, annealing to one and displacing the other. Strand has many applications in nanoengineering fundamental biological processes such as homologous recombination repair of double breaks. previously been studied bulk; however, slow, bimolecular toehold formation step obscures kinetics bulk studies. Here, we des...
INTRODUCTION AND OBJECTIVES The zinc-finger transcription factor Krϋppel-like factor 2 (KLF2) transduces blood flow into molecular signals responsible for a wide range of responses within the vasculature. KLF2 maintains a healthy, quiescent endothelial phenotype. Previous studies report a range of phenotypes following morpholino antisense oligonucleotide-induced klf2a knockdown in zebrafish. Ta...
Supplementary Methods Paired end ditags (PETs) Gene identification signature (GIS) analysis is a sequencing and mapping strategy that allows for the high-throughput demarcation of gene transcription boundaries, i.e. the 5’ and 3’ gene termini (Ng, et al., 2005). The GIS analysis procedure that produced the data we analyzed started with the isolation of polyA+ RNA from cells lines subject to dif...
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