0.8/0.2, acrylamide: bis :diallyltartardiamide, 0.5 x TBE [ 1 X TBE = 0.089 M-Tris base, 0.089 M-boric acid, 0.002 MEDTA) which had been pre-electrophoresed overnight at 75 V in 0.5 XTBE buffer. Protein standard markers were loaded in parallel lanes and the gel was electrophoresed for 1500 V h. The gel was stained with Coomassie Brilliant Blue R to visualize the protein standards, dried and exp...

Small mountain lakes are natural archives for understanding long-term and anthropogenic impact on the environment. This study focused (last ca. 13 000 years) vegetation changes sedimentary processes in catchment area of Lake Planina pri jezeru (1430 m a.s.l.) by using mineralogical, geochemical palynological methods. Palynological results suggest that regional between 12 900 11 700 cal a bp was...

The major cause of sea-level change during ice ages is the exchange of water between ice and ocean and the planet's dynamic response to the changing surface load. Inversion of ∼1,000 observations for the past 35,000 y from localities far from former ice margins has provided new constraints on the fluctuation of ice volume in this interval. Key results are: (i) a rapid final fall in global sea l...

Background: Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m 1 and Cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m 3. Methods:The Cuc m 3 cDNA has been characterized by rapid amplification of cDNA ends (RACE), which ...

OBJECTIVES It was suggested that glucocorticoid exerts cell-specific effects on thyrotropin-releasing hormone (TRH) gene expression at the transcriptional level. Although there is no typical palindromic glucocorticoid response element (GRE) on the rat TRH gene promoter, a perfect GRE half-site is found between -205 bp and -211 bp. We investigated whether the segment between -242 bp and -200 bp ...

Pisang tongkat langit (Musa troglodytarum L.) is a banana cultivar originated from Eastern Indonesia. Previous studies showed that ACS1 and ACO1 are genes play an important role in ethylene biosynthesis. However, characterization analysis of M. have not been reported. This study aims to isolate characterize the gene expression profile during fruit ripening process. Gene fragment isolation was p...

background: melon (cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. to date, two allergen molecules, cuc m 1 and cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of cuc m 3. methods:the cuc m 3 cdna has been characterized by rapid amplification of cdna ends (race), which rev...

Mycoplasmas are important  avian  pathogens,  which  can  cause  both  respiratory  disease  and  synovitis  in  poultry  that  result  in  considerable  economic  losses  to  the  poultry  industry  all  over  the  world.  The  aim  of  this  study  was  to  determine  the  prevalence  of  Mycoplasma  gallisepticum  and  Mycoplasma  synoviae  infections  among  commercial  poultry  flocks  in ...

b ackground: due to the lack of a suitable and economic test for the analysis of the polymorphism at codon 167, we developed a new pcr-rflp technique, based on a modified forward primer (ut- hc167 mf-primer), to identify simultaneously the snps at codons 167 and 200 of isotype 1 β-tubu- lin gene of haemonchus contortus . m ethods: there already are several safe and easy methods for identificati...