The cost of 3D printing has reduced dramatically over the last few years and is now within reach of many scientific laboratories. This work presents an example of how 3D printing can be applied to the development of custom laboratory equipment that is specifically adapted for use with the novel brain tissue clearing technique, CLARITY. A simple, freely available online software tool was used, a...

This work aims at three goals: first, to define a set of statistical parameters and plan structures for a 3D pretreatment thoracic and prostate intensity-modulated radiation therapy (IMRT) quality assurance (QA) protocol; secondly, to test if the 3D QA protocol is able to detect certain clinical errors; and third, to compare the 3D QA method with QA performed with single ion chamber and 2D gamm...

Single cell manipulation technology has been widely applied in biological fields, such as cell injection/enucleation, cell physiological measurement, and cell imaging. Recently, a biochip platform with a novel configuration of electrodes for cell 3D rotation has been successfully developed by generating rotating electric fields. However, the rotation platform still has two major shortcomings th...

We have developed a simple microfluidic device for generating stable concentration gradients in 2D and 3D environments. The device, termed the Ladder Chamber, uses a two-compartment diffusion system to generate steady state gradients across flow-free channels that connect the source and sink channels. To demonstrate the utility of the Ladder Chamber for cell migration, neutrophil chemotaxis was...

A three-dimensional (3D) particle-in-cell (PIC) code based on the template potential concept [1-6] has been upgraded by inclusion of a fully parameterized model for 3D space charge force calculations. The dynamics of a bunched 3D beam were studied for FODO channels having a conducting chamber with an arbitrary elliptical symmetry. The simulation results were in a good agreement with conventiona...

This study was conducted to determine the use of three-dimensional ultrasonography (3DU) in ocular imaging of the dog and measuring of its optical axis. 12 healthy mixed-breed dogs including 6 males and 6 females were studied. 3DU of the eyes were done using a 5–12 MHz linear trapezoid transducer. 3DU of the eyes were evaluated and the normal optical long axis through a line between the cornea ...

While live 3D high resolution microscopy techniques are developing rapidly, their use for biological applications is partially hampered by practical difficulties such as the lack of a versatile sample chamber. Here, we propose the design of a multi-usage observation chamber adapted for live 3D bio-imaging. We show the usefulness and practicality of this chamber, which we named the UniverSlide, ...

Objective-  To determine the possibility of three-dimensional ultrasonography (3DUS) and measurement of optical nerve sheet by this method. Design- Descriptive study. Animals- 10 mixed normal dogs (5males, 5 females), age 1-1.5 year, and weighting 15±5 kg Procedure- 3D ultrasounds of the eyes were evaluated and the normal optical nerves in 3DUS images were measured using Volouson 730 pro....

Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on slide and have adapted this to our electrotaxis chamber, thereby offering a no...

Previous work demonstrated the power of three-dimensional (3D) magnetic resonance microscopy (MRM) to follow complicated morphologic development in the embryonic cardiovascular system. In this study we describe a new dual-contrast method for specimen preparation that combines perfusion fixation and immersion in fixative with macro- and small molecular gadolinium agents to provide enhanced defin...