The tumor suppressor p53 and miRNAs are linked through a complex network. Several miRNAs modulate p53 expression, while p53 regulates the transcription and/or biogenesis of several other miRNAs. Here, we report the development of a cell-based assay used with a library of human miRNA mimics in a high-throughput screen for miRNAs that modulate p53 expression. Overexpression of miRNA (miR)-542-3p ...

Joseph Kwan1†, Damian Jenkinson2, Mike Vassallo3, Nicola Englyst4 & V Hugh Perry5 †Author for correspondence 1Royal Bournemouth Hospital, Department of Medicine for the Elderly, Castle Lane East, Bournemouth, BH7 7DW, UK Tel.: +44 1202 704 590; Fax: +44 1202 704 542; E-mail: [email protected] 2Tel.: +44 1202 705 292; Fax: +44 1202 704 542; E-mail: damian.jenkinson@ rbch.nhs.uk 3Tel.: +44 ...

The zebrafish (Danio rerio) is a useful vertebrate model for use in cardiovascular drug discovery. The present study aimed to construct optimized methods for the study of intravascular lipid metabolism of zebrafish. The lipophilic dye, Oil Red O, was used to stain fasting zebrafish one to eight days post-fertilization (dpf) and to stain 7-dpf zebrafish incubated in a breeding system containing ...

Endometrial decidualization represents an essential step for the successful implantation of the embryo; however, the molecular mechanism behind this differentiation process remains unclear. This study aimed to identify novel microRNAs (miRNAs) involved in the regulation of decidual gene expression in human endometrial stromal cells (HESCs). An in vitro analysis of primary undifferentiated and d...

Aim Endometriosis is defined as the presence of endometrial glandular and stromal cells outside of the uterine cavity. A previous study reported that microRNA (miR)-542-3p plays a critical role in eutopic endometrial decidualization. This study aims to clarify the potential role of miR-542-3p and the target gene, IGFBP-1 (insulin-like growth factor-binding protein 1), in the impairment of the d...

Isolation of Proteins from Fermentation Broth of high Cell Density with a new Cross Flow Filtration E.T. Davies, M. Tellez and M. Unger University of Georgia Life Sciences Building, 1057 Green Street, Athens GA 30602, Tel.: 706 542 1035, Fax: 706 542 1077, [email protected] [email protected] [email protected]

Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000. The 5-year survival rate of patients with osteosarcoma has significantly improved over time; however, there remains a significant proportion of patients that respond poorly to chemotherapy. An ...

19 INITIAL INVESTIGATION OF ANALYTICAL EXTRACTION TECHNIQUES FOR THE DETERMINATION OF BIOAVAILABILITY OF PESTICIDES IN SOIL B.K. Kramer, P.B. Ryan, and D.L. MacIntosh Department of Chemistry, Emory University, Atlanta, GA 30322; Phone: (404)727-9259; Fax: (404)727-8744. Rollins School of Public Health, Emory University, Atlanta, GA 30322; Phone: (404)727-3826; Fax: (404)727-8744. Environmental ...

In earlier studies 1 the electrical behavior of Nitella conformed to the equat ions of Nerns t and of Henderson bu t recently, in a different set of cells, ~ we have found some appa ren t exceptions. These exceptions are i l lustrated in Fig. 1. The theoret ical curve shows the approx imate change in P.D. which KC1 would produce if the values were due entirely to diffusion potent ia l in the pr...

Since hypophosphatasia was first described (Rathbun, 1948), other cases which have been recognized have raised the possibility that this defect of alkaline phosphatase is an inherited inborn error of metabolism. Schneider and Corcoran (1950) first suggested that this was an inherited disorder when two cases were found in one family, and this was corroborated by Sobel, Clark, Fox and Robinow (19...