The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) is currently spreading among humans, making development of effective MERS vaccines a high priority. A defined receptor-binding domain (RBD) in MERS-CoV spike protein can potentially serve as a subunit vaccine candidate against MERS-CoV infections. To identify an ideal vaccine candidate, we have constructed five different ...

Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human haematopoietic stem cells from heterogeneous cord blood mononuclear ...

background: in the previous study, we have shown that the presence of a allele at position -588 in aγ-globin gene was highly frequent and closely associated with fetal hemoglobin elevation among β-thalassemia intermedia patients. therefore, we decided to investigate whether this allele (a allele at -588) could result in an increase in aγ-globin gene expression to ameliorate the severity of the ...

6.2. Orthogonality versus Independence 584 6.3. Cross-sensitivity and Diversity 585 6.4. Multiple Roles of Redundancy 585 7. Data Preprocessing 586 7.1. Baseline Correction 586 7.2. Scaling 587 7.2.1. Global Techniques 588 7.2.2. Local Techniques 588 7.2.3. Nonlinear Transforms 588 8. Drift Compensation 588 8.1. Univariate Drift Compensation 589 8.2. Multivariate Drift Compensation 589 9. Featu...

Background: In the previous study, we have shown that the presence of A allele at position -588 in γ-globin gene was highly frequent and closely associated with fetal hemoglobin elevation among β-thalassemia intermedia patients. Therefore, we decided to investigate whether this allele (A allele at -588) could result in an increase in γ-globin gene expression to ameliorate the severity of the di...

Background: In the previous study, we have shown that the presence of A allele at position -588 in γ-globin gene was highly frequent and closely associated with fetal hemoglobin elevation among β-thalassemia intermedia patients. Therefore, we decided to investigate whether this allele (A allele at -588) could result in an increase in γ-globin gene expression to ameliorate the severity of the di...

The amylase of waxy and non-waxy seeds. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 573 The amylase of waxy and non-waxy pollen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 577 Resultsof tests with S, W, and S, W, pollen extracts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 579 Results of tests wit...