Six dissociable groups exist in tetrahydrofolic acid in the range Ho -3.0 to pH 14. The assignments in tetrahydrofolic acid by titrimetric and spectrophotometric comparisons with model compounds, +uninobenzoylglutamic acid and 2-amino-6,7-dimethyl-5,6,7,8-tetrahydro-4-pteridinone, are as follows: acidic ionization of amide, pK’ 10.5; Ns, pK’ 4.82; y-carboxyl, pK’ 4.8; cr-carboxyl, pK’ 3.5; N1, ...
