Tuberculosis (TB) is one of the oldest disease known to humanity and among deadliest epidemics in top 10 causes death worldwide. The incidence tuberculosis India has dramatically increased contributing it Human Immunodeficiency Virus (HIV). Various diagnostic methods are available for detection tubercular bacilli. Only full proof criteria diagnosis TB demonstrate bacilli from affected tissue. T...

background and objective: bcg vaccination is used in many countries with a high prevalence of tb to prevent childhood tuberculosis meningitis and miliary disease. local and systemic sideeffects are associated with bcg vaccine. the most critical reaction is disseminated bcg infection which occurs in mostly immunodeficient patients. materials and methods: we performed 4 autopsies during 2001-2003...

A dual-staining procedure for acid-fast bacilli was found to have poor correlation with the Ziehl-Neelsen and auramine-rhodamine staining techniques.

the aim of this study was to investigate the histopathology of avian tuberculosis in naturally infected domestic pigeons (columba livia var. domestica) with mycobacterium aviumsubsp.avium. avian tuberculosis is one of the most important diseases that affect all species of birds, and is most often caused by mycobacterium avium and mycobacterium genavense. eighty out of more than 600 pigeons were...

This paper presents the finding of the possible cause of the high false-positive rate in acid-fast staining in histological examinations. Using acid-fast staining, culture, and PCR, acid-fast bacilli were detected in 83.7% of 49 hospital tap water samples and nontuberculous mycobacteria (NTM) were detected in 20.4% of the same 49 samples. The 10 NTM isolates were also identified to the species ...

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...

background: tuberculosis (tb) is an increasing public health concern worldwide. on a global scale it has a devastating impact in developing nations. genital tb, an extrapulmonary form, is not uncommon particularly in areas where pulmonary tb is prevalent. genital tb may be asymptomatic or may even masquerade as other gynaecological conditions; hence, diagnosis requires a high degree of suspicio...

The lesser degree of acid-fastness of lepra bacilli as compared with tubercle bacilli appears in a disagreeable manner in the difficulty of demonstrating the organisms of leprosy in paraffinized tissues (1). Faraco (2) showed that by ordinary methods of demonstrating acid-fast organisms the lepra bacilli are often not acid-fast, are not differentiated and may be stained by the counterstain. He ...

Reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. By the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly stained. Moreover, non-deparafinized sections can be used for the periodic acid-car...