background: escherichia coli (e. coli) is the most extensively used host for the production of recombinant proteins. however, most of the eukaryotic proteins are typically obtained as insoluble, misfolded inclusion bodies that need solubilization and refolding. reteplase as a highly disulfide-bonded recombinant protein is an example of difficult to express protein in e. coli. methods: in this s...

objective: rna interference (rnai) is the most potent technique for gene silencing in eukaryotic cellular system at transcriptomic level. genetic disorders and cancers are important targets for therapeutic development of this technique. in order to bypass the temporary dpwnregulation by sirna, a new generation of shrna named shrnamir has developed. silencing construct with structure similar to ...

stroke is an important cause of disability and death worldwide, with the majority of strokes occurring in older people. thrombolysis with recombinant tissue plasminogen activator (r-tpa) is the approved treatment for acute ischemic stroke. a major concern of physicians, who treat acute ischemic stroke with recombinant tissue plasminogen activator (r-tpa,) is the risk of intracerebral hemorrhage...

in this research, aluminizing packs containing naf and nh4cl activators were used for aluminizing of pure titanium samples at 1100 ºc. the results showed that the layers of tial3, tial2, γ-tial, α2-ti3al, and α-solid solution are formed on titanium samples in both cases. however, the weight gain and the thickness of tial3 layer in treated samples with nh4cl activator are greater than those when...

background: firoozgar comprehensive stroke center started up as the first organized care unit in the country in 2014; this study was performed to investigate quality indicators such as reduction in mortality, morbidity and hospital stay.   methods: two groups of ischemic stroke patients were compared. the first group had been admitted in general neurology ward (non-stroke unit patients) and the...

objective: development of high producing mammalian cell lines is a major bottleneck in manufacturing of recombinant therapeutic proteins. this study examines the effect of using the matrix attachment region from the human interferon beta gene in combination with promoter activation strategy with e1a 13s protein on human tissue plasminogen activator (t-pa) expression in chinese hamster ovary (ch...

results a total of 46 patients participated in this study. the significant differences between recovering patients and the patients who died, regarding aspartate aminotransferase (ast), creatine phosphokinase (cpk), lactate dehydrogenase (ldh), prothrombin time (pt), activated partial thromboplastin time (aptt), and thrombocyte and fibrinogen levels, were consistent with many clinical studies i...

Quorum sensing controls the luminescence of Vibrio fischeri through the transcriptional activator LuxR and the specific autoinducer signal produced by luxI. Amino acid sequences of these two genes were analyzed using bioinformatics tools. LuxI consists of 193 amino acids and appears to contain five α-helices and six ß-sheets when analyzed by SSpro8. LuxI belongs to the autoinducer synthetase fa...

quorum sensing controls the luminescence of vibrio fischeri through the transcriptional activator luxr and the specific autoinducer signal produced by luxi. amino acid sequences of these two genes were analyzed using bioinformatics tools. luxi consists of 193 amino acids and appears to contain five α-helices and six ß-sheets when analyzed by sspro8. luxi belongs to the autoinducer synthetase fa...