Quantitation of DNA adducts in human tissues has been achieved with highly sensitive techniques based on adduct radiolabeling, antisera specific for DNA adducts or modified DNA, and/or adduct structural characterization using chemical instrumentation. Combinations of these approaches now promise to elucidate specific adduct structures and provide detection limits in the range of 1 adduct/10(9) ...

The mechanism of reaction of cyclic monoimides with oxiranes was established based upon kinetic studies and product analysis. It has been established that the reaction proceeds through initial formation of an adduct of imide and triethylamine. The crucial bond in adduct has ionic character; in non-aqueous solvents it is present as ion pair, while in water the adduct dissociate and free ions are...

Macrocyclic tris-malonates incorporating cleavable di-tert-butylsilylene protecting groups have been prepared by a stepwise approach and used for the regioselective tris-functionalization of C60. Fullerene tris-adducts with an e,e,e addition pattern thus obtained. The bridging cleaved to liberate terminal alcohol functions fullerene tris-adduct. This key building block has further functionalize...

4-Dimethylaminoazobenzene (DAB, methyl yellow, or butter yellow), a human carcinogen, has been banned for use in foods since 1988. In 2014, DAB adulteration in Tofu occurred in Taiwan. We hypothesize that DAB can form [DAB•SBP]adduct adduct with soybean protein (SBP) which could damage Gastro-Duodenal-Hepatic axis. Sprague-Dawley rats gavage fed [DAB•SBP]adduct adduct revealed severely reduced ...

Benzo(a)pyrene (BP) and other polycyclic aromatic hydrocarbons (PAH) are ubiquitous environmental pollutants and are suspected to be carcinogenic in man. The in vivo formation of BP metabolite-DNA adducts has been characterized in a variety of target and nontarget tissues of mice and rabbits. Tissues included were lung, liver, forestomach, colon, kidney, muscle, and brain. The major adduct iden...

the formation constants and gibbs free energy for the interaction of rnsnclm (n = m = 2, r = me, ph, n-bu or n = 4, m = 0, r = ph) compounds as acceptors, with ml complexes (h2l = n,n'-bis(salicylidene)-2-aminobenzylamine (salabza-h2) and its derivatives, m = mn2+ or zn2+), as donors in n,n'-dimethylformamide (dmf) as solvent were studied. these parameters were measured by using uv-vis spectrop...

The mechanistic analysis of catalyst-controlled regiodivergent aza-Morita-Baylis-Hillman reactions vinylcyclopentenone is described. DABCO-catalyzed reaction gives the α-adduct exclusively (red reaction) and DMAP-catalyzed produces γ-adduct selectively (blue reaction). revealed that rate-determining step (RDS) α-selective Mannich-type C−C bond formation step, whereas RDS γ-selective seems to be...

Due to difficulties in conducting epidemiological studies, most estimates of cancer risk are based on data from animal bioassays. Extrapolation of cancer risk estimates in animals to humans requires an assumption of equal potency across species based on the average daily dose. The purpose of this paper is to examine the ability to predict tumor incidence across species from DNA adduct concentra...

Detection of the O6-Carboxymethylguanine DNA Adduct in Colorectal Cancer Using a Graphene Field-Effect Transistor-Based Biosensors

The major adduct formed on acid hydrolysis of calf thymus DNA which has been reacted with 8,9-dichloro-8,9-dihydroaflatoxin B1, a chemical model of the ultimate carcinogen 8,9-dihydro-8,9-epoxyaflatoxin B1 (AFB1-epoxide), has been characterized by proton nuclear magnetic resonance and fast atom bombardment mass spectroscopy. This adduct has been identified as an N7-substituted guanine adduct an...