The adenine nucleotides, adenosine diphosphate, adenosine triphosphate, (ATP), and the methylene-bridge analogues are inhibitors of rickettsial adsorption to and the hemolysis of sheep erythrocytes. Other nucleotides, adenosine monophosphate, cyclic adenosine monophosphate, cytosine triphosphate, and guanosine triphosphate, are without effect. Adsorption and hemolysis require the generation of ...

Extracellular ATP mediates proinflammatory and antiproliferative effects via activation of P2 nucleotide receptors. In contrast, its metabolite, the nucleoside adenosine, is strongly immunosuppressive enhances tumor proliferation metastasis. The conversion to adenosine catalyzed by ectonucleotidases, which are expressed on immune cells typically upregulated cells. present study, we identified s...

The energy demand of cardiomyocytes changes continuously in response to variations cardiac workload. Cardiac excitation-contraction coupling is fueled primarily by adenosine triphosphate (ATP) production oxidative phosphorylation mitochondria. rate mitochondrial metabolism matched the ATP consumption cytosol parallel activation calcium (Ca 2+ ) and diphosphate (ADP). During workload transitions...

It has been proposed that extracellular ATP inhibits synaptic release of glutamate from hippocampal CA1 synapses after its catabolism to adenosine. We investigated the possibility that at least part of this effect is mediated by ATP itself acting on P2Y receptors. ATP and various analogs decreased the amplitude and duration of glutamate-mediated excitatory postsynaptic potentials in all tested ...

Zr(IV) can form phosphate and Zr(IV) (-PO₃2--Zr4+-) complex owing to the high affinity between Zr(IV) with phosphate. Zr(IV) can induce the aggregation of gold nanoparticles (AuNPs), while adenosine triphosphate(ATP) can prevent Zr(IV)-induced aggregation of AuNPs. Herein, a visual and plasmon resonance absorption (PRA)sensor for ATP have been developed using AuNPs based on the high affinity be...

Solutions of adenosine triphosphate (ATP) were injected into the left coronary artery of isolated nonperfused dog hearts kept for 30 to 180 minutes at temperatures varying from 4 to 34°C. The amount of ATP administered varied from 0.5 to 7 /xmoles/g heart. The left ventricles of the ATP-treated hearts had a higher content of adenine nucleotides and of phosphocreatine than did the left ventricle...

The 26 S proteasome complex is thought to catalyse the breakdown of ubiquitinated proteins within eukaryotic cells. In addition it has been found that the complex also degrades short-lived proteins such as ornithine decarboxylase in a ubiquitin-independent manner. Both proteolytic processes are paralleled by the hydrolysis of ATP. Here we show that ATP also affects the hydrolytic activity towar...

Mammals experience some degree of hypoxia during their lifetime. In response to hypoxic challenge, mammalian cells orchestrate specific responses at transcriptional and posttranslational level which lead changes in the purine metabolites order cope with threatening conditions. The aim this study was evaluate enzymes involved metabolism human muscle Muscle culture were exposed enzymatic activity...

A styrylpyridine-containing cyclophane with diethylenetriamine linkers is presented as a host system whose association representative nucleotides was examined photometric and fluorimetric titrations. The spectrometric titrations revealed the formation of 1:1 complexes log Kb values in range 2.3–3.2 for pyrimidine TMP (thymidine monophosphate), TTP triphosphate) CMP (cytidine monophosphate) 3.8–...

Arginyl dipeptides like Arg-Ser, Arg-Ala, and Arg-Gly are salt-taste enhancers can potentially be used to reduce the salt content of food. The l-amino acid ligase RizA from B. subtilis selectively synthesizes arginyl dipeptides. However, industrial application is prevented by high cost cofactor adenosine triphosphate (ATP). Thus, a coupled reaction system was created consisting acetate kinase (...