نتایج جستجو برای: affinity

تعداد نتایج: 86334  

Journal: :iranian journal of immunology 0
fatemeh hajighasemi department of immunology, school of public health, tehran university of medical sciences ali akbar saboor-yaraghi department of immunology, school of public health, tehran university of medical sciences fazel shokri department of immunology, school of public health, tehran university of medical sciences

background: the affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as elisa. affinity of most igg specific mabs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

Journal: :iranian biomedical journal 0
اسکندر امیدی نیا eskandar omidinia حشمت ا... طاهرخانی heshmatollah taherkhani yasuhisa asano شهره خاتمی shohreh khathami علی رضا عمومی alireza omumi عطاءا... غدیری ata-allah ghadiri

cloning and expression of the l-phenylalanine dehydrogenase gene, from b. sphaericus in e. coli were done. the gene was cloned in the vector pet16b and transformed into e. coli bl21 (de3). the functional form of the l-phenylalanine dehydrogenase enzyme was purified by affinity purification techniques, taking advantage of the ability of this enzyme to bind to the nucleotide site affinity dye, re...

Journal: :iranian journal of medical sciences 0
fan chen department of biochemistry and molecular biology, life sciences school of hubei university, wuhan, china si-chong chen evolution and ecology research centre, school of biological, earth and environmental sciences, the university of new south wales, sydney, australia jing zhou clinical laboratory, wuhan tuberculosis dispensary, wuhan health bureau, wuhan, china zhi-de chen department of biochemistry and molecular biology, life sciences school of hubei university, wuhan, china fang chen department of biochemistry and molecular biology, school of basic medical science, wuhan university, wuhan, china

hepatitis c virus (hcv) encodes two envelope glycoproteins, e1 and e2. our previous work selected a specific aptamer ze2, which could bind to e2 with high affinity, with a great potential for developing new molecular probes as an early diagnostic reagents or therapeutic drugs targeting hcv. in this study, the binding sites between e2 and aptamer ze2 were further explored. e2 was truncated to 15...

Journal: :Nature Reviews Drug Discovery 2002

Journal: :Between the Species: An Online Journal for the Study of Philosophy and Animals 1985

Journal: :journal of physical & theoretical chemistry 2011
mohammad hossien ghorbani reza fazaeli arash ghoorehian

the term electronegativity .was introduced by linus pauling, who characterized on the basis ofthermodynamic data from the energies of the single bonds. in present study, the density functional theory(dft) was used to calculate electronegativity of atoms. the base of calculation is similar to pauling andmulliken methods. the results indicate that the largest value of electronegativity for fluori...

Journal: :research in molecular medicine 0
zahra moradi nebrin immunology research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran jafar majidi tabriz autonomous university of medical sciences, tabriz university of medical sciences, tabriz, iran leili aghebati maleki immunology research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran tohid kazemi department of immunology, faculty of medicine, tabriz university of medical sciences, tabriz, iran jalal abdolalizadeh department of immunology, faculty of medicine, tabriz university of medical sciences, tabriz, iran somaieh dadashi immunology research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran

background: the ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis. the aim of this study was purification of mouse igg2a and production of polyclonal antibody against purified mouse igg2a subclass. materials and methods: mouse igg2a was purified by proa affinity. verification method of the purified antibody was sds-...

Journal: :iranian journal of nuclear medicine 2012
cnb harisankar

parathyroid adenoma involving a single parathyroid gland is the underlying cause of 80-85% of primary hyperparathyroidism (phpt). skeletal system is significantly affected by phpt. brown tumors are known to have affinity for 99mtc-mibi. we report a rare case of phpt presenting with diffuse bony pain, high calcium level and significantly elevated alkaline phosphatase level. 99mtc-pertechnetate/9...

Fan Chen, Fang Chen Jing Zhou Si-Chong Chen Zhi-De Chen

Hepatitis C Virus (HCV) encodes two envelope glycoproteins, E1 and E2. Our previous work selected a specific aptamer ZE2, which could bind to E2 with high affinity, with a great potential for developing new molecular probes as an early diagnostic reagents or therapeutic drugs targeting HCV. In this study, the binding sites between E2 and aptamer ZE2 were further explored. E2 was truncated to 15...

Protein specific aptamers are highly applicable affinity ligands in different fields of research and clinical applications. They have been developed against various targets, in particular, bio-macromolecules such as proteins. Among human proteins, the coagulation factors are the most attractive targets for aptamer selection and their specific aptamers had valuable characteristics in therapeutic...

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