Acid-soluble collagen (ASC) from the fish skin of African catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic cod (Gadus morhua) was extracted and characterized. The ASC extraction yield was 75%, 73% and 68%, respectively. The denaturation and melting temperatures of African catfish ASC (29.3°C and 100.0°C) were significantly higher than ASC of Salmon and Baltic cod (20.6°C and 90.5°C...

In several waterbreathing fish species, hypoxia induces a decrease in plasma free fatty acid (FFA) levels as opposed to an increase in air-breathing mammals. We hypothesised that this change is coupled to the mode of breathing. Therefore, we followed the metabolic response of cannulated air-breathing African catfish to an 8-h asphyxia period. The hematocrit and hemoglobin increased significantl...

background: pseudomonas spp. are of important food spoiling bacteria. they present around the world and are isolated from diverse sources. the main objective of this survey was to detect pseudomonas spp. in fresh chicken and fish obtained from markets of assiut city, egypt.  methods: in this cross-sectional survey, 50 samples of fresh raw chicken and 50 samples of fresh raw fish meat were rando...

LH and FSH are heterodimeric glycoprotein hormones, composed of a common alpha-subunit non-covalently associated with a hormone-specific beta-subunit. Repeated efforts to isolate catfish FSH (cfFSH) have not been successful and only catfish LH (cfLH) has been purified from catfish pituitaries. Recently, however, we succeeded in cloning the cDNA encoding the putative cfFSHbeta; the cDNAs for the...

The aim of the present study is to evaluate the cytotoxic and genotoxic effects of silver nanoparticles (Ag-NPs) towards African catfish (Clarias gariepinus). Adult male catfish was exposed to 0, 25, 50, and 75 mgl–1 Ag-NPs for two weeks. Exposure to Ag-NPs exerted an increase in mortality rate and behavioural changes compared to control. A fluorescent microscopy examination was used to assess ...

In African catfish, two gonadotropin-releasing hormone (GnRH) peptides have been identified: chicken GnRH (cGnRH)-II and catfish GnRH (cfGnRH). The GnRH receptors on pituitary cells producing gonadotropic hormone signal through inositol phosphate (IP) elevation followed by increases in intracellular calcium concentration (¿Ca(2+)(i)). In primary pituitary cell cultures of male African catfish, ...

AIM An experiment was conducted to evaluate the use of Bacillus thuringiensis (Bt) as a probiotic to enhance the cellular innate immune response of the African catfish (Clarias gariepinus) challenged with a bacterial fish pathogen, Aeromonas hydrophila. MATERIALS AND METHODS The bacterium (Bt) was administered orally at three different dietary doses of 0.5 x 10(7) (T2), 1.0 x 10(7) (T3) and 1...

Catfish is one of the most important freshwater fish farming commodities in Indonesia. Higher catfish production can be achieved by cultivating transgenic carrying growth hormone (GH) gene African (Clarias gariepinus GH, CgGH). This research focuses on analysis presence CgGH G1, G2, and G3 mutiara broodstock, as an indication stable inheritance. was isolated using RNeasy mini kit RT-PCR. RT-PCR...