Understanding the structural basis of protein redox activity is still an open question. Hence, by using a structural genomics approach, different albumins have been chosen to correlate protein structural features with the corresponding reaction rates of thiol exchange between albumin and disulfide DTNB. Predicted structures of rat, porcine, and bovine albumins have been compared with the experi...

Serum albumins appeared in early vertebrates as a result of gene triplication. Their canonical structure supported by a conservative set of disulfide bridges is maintained in all mammals, but the changes in sequence are highly correlated with the evolution of each species. Serum albumin is the main carrier for metabolites, hormones and drugs in the blood. It collects drugs from the place of app...

The present study aimed to investigate the influence of albumin structure and gold speciation on the synthesis of gold nanoparticles (GNPs). The strategy of synthesis was the addition of HAuCl4 solutions at different pH values (3-12) to solutions of human and bovine serum albumins (HSA and BSA) at the same corresponding pH values. Different pH values influence the GNP synthesis due to gold spec...

Binding of a newly synthesized indanedione derivative, 2-(2-hydroxy-3-ethoxybenzylidene)-1,3-indanedione (HEBID), to human and bovine serum albumins (HSA and BSA), under simulated physiological conditions was monitored by fluorescence spectroscopy. The binding parameters (binding constants and number of binding sites) and quenching constants were determined according to literature models. The q...

The effect of protein and amino acid charges was studied on protein synthesis, proteases and cytochrome oxidase in the kidney homogenates of frog. The gamma globulins and basic proteins, decreased the protein synthesis as well as the protein degradation while the albumins and acidic proteins showed an opposite effect, suggesting a high protein turnover rate in the kidney of frog. Lysine and glu...

Castor plant (Ricinus communis L.) produces a unique seed oil with numerous industrial applications. However, castor seed contains toxin ricin and hyper-allergenic 2S albumins detrimental to castor grower and processor. Our project goal is to develop a safe source of castor oil through genetic engineering. The general approach is to generate a safe castor crop by blocking expression of the rici...

The interaction of pinostrobin (PS), a multitherapeutic agent with serum albumins of various mammalian species namely, goat, bovine, human, porcine, rabbit, sheep and dog was investigated using fluorescence quench titration and competitive drug displacement experiments. Analysis of the intrinsic fluorescence quenching data revealed values of the association constant, K(a) in the range of 1.49 -...