INTRODUCTION Mesenchymal stem cells (MSCs) are defined as cells that can differentiate into multiple mesenchymal lineage cells. MSCs were obtained from various sources; bone marrow, umbilical cord blood, adipose tissue and synovium etc. It has been reported that MSCs isolated from the bone marrow showed variation in size, morphology and level of alkaline phosphatase activity among clones isolat...

We have studied five patients who have exhibited an unusual alkaline phosphatase isoenzyme (ALP EC 3.1.3.1) migrating in an ultrafast position electrophoretically on cellulose acetate. This ALP isoenzyme has been identified in patients with benign and malignant liver diseases. In addition, a number of these patients exhibited a regular ALP liver isoenzyme and a fast (preliver) ALP isoenzyme in ...

The liver is one of the organs involved in the multiorgan failure that occurs in sickle cell disease, the pathophysiology of liver disease in this condition is complex because of the interrelated multifactorial causes. Liver dysfunction was assessed in both paediatric and adult sickle cell disease patients in the steady state. The transaminases and alkaline phosphatase were analysed by automati...

Alkaline phosphatase (ALP) activity was detected using bovine serum albumin (BSA)-functionalized manganese-doped zinc sulfide (Mn–ZnS) quantum dots (QDs), and the phosphate substrate vitamin-B 6 cofactor pyridoxal 5′-phosphate (PLP).

Fractionation of serum alkaline phosphatase by either biochemical or electrophoretic techniques is often insufficient to determine the source of the elevated serum enzyme. In 31 unselected patients with high serum alkaline phosphatase a simultaneous determination of serum gamma glutamyl transpeptidase and urinary hydroxyproline excretion rates was performed, in addition to urea denaturation and...

To measure changes in bone alkaline phosphatase (EC 3.1.3.1) activity in serum as a function of duration of pregnancy, we adapted our existing alkaline phosphatase (ALP) isoenzyme assay (which has been used to measure bone, hepatic, and intestinal ALP activities in serum, in the absence of placental ALP) to allow quantification of individual ALP isoenzyme activities in the presence of placental...

Enzymatic and immunological properties of alkaline phosphatase [ALP; orthophosphoric monoester phosphohydrolase, alkaline optimum, EC 3.1.3.1.] in the human dental pulp were investigated. In inhibition and thermal inactivation studies, dental pulp ALP showed properties of universal-type ALP (kidney/bone/liver type). Dental pulp ALP cross-reacted with polyclonal and monoclonal antibodies against...

In this work, automatic pH changing system on lab-on-paper devices for enzyme-linked immunosorbent (ELISA) has been developed in order to improve their sensitivity and make the technique much more applicable to real samples. The most widely used enzyme in immunoassay are alkaline phosphatase (ALP) and peroxidase. ALP-conjugated secondary detection with BCIP/NBT substrate provides high sensitivi...

The reduction of tetrachloroaurate ions (AuCl(4)(-)) with 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) was used to detect alkaline phosphatase (ALP) activity.

Administration of mercuric chloride (HgCl2; 5.0 mg/kg body weight) to male Swiss albino-mice resulted in significantly higher levels of testicular acid phosphatase (ACP) and alkaline phosphatase (ALP) activities as compared to control. In combination group where S. fusiformis (800 mg/kg body weight) was given before and after HgCl2 treatment, the mercury induced toxicity reduced in terms of dec...