An alkaline phosphatase (EC 3.1.3.1) of the placental type was isolated from a seminoma type of human testicular cancer tissue and was purified to homogeneity by sulfate-mediated chromatography on a column of Cibacron Blue Sepharose 4B. The purified enzyme had a specific activity of 40.6 kU per gram of protein and was obtained in a yield of 37%. The purification procedure used was simple and ec...

A neutron activation analysis method with pre-irradiation separation and post-irradiation purification is presented for the determination of 129I content and 129I/127I ratio in some environmental samples, such as seawater, seaweed, grass and soil. An evaporation concentration followed by solvent extraction was used for the separation of iodine from seawater, and alkaline ashing or alkaline fusi...

The present study utilized different methods for purification of alkaline protease by using ammonium sulphate and acetone. Ammonium sulphate was found the best purifying agent and gave 109.09 fold purification of protease with 49.7% yield at 60% saturation. Dialysis was performed by using phosphate and Tris-HCl buffer and phosphate buffer was optimized to good dialyzing agent at pH 8.0.

In 1935, Shay and Gershon-Cohen showed that luminal perfusion of the duodenum of human subjects with sodium bicarbonate solution produced a rapid emptying of a barium sulphate meal from the stomach. More recently it was shown by Brown et al. (1966) that, in the dog, the instillation of alkaline solutions into the duodenum also increased motor activity in denervated, transplanted gastric pouches...

Purified phosphatidylinositol-specific phospholipase C from Staphylococcus aureus released a substantial proportion of the total alkaline phosphatase activity from a wide range of tissues from several mammalian species. Co-purification of the phospholipase C and alkaline phosphatase-releasing activities and the inhibition of both these activities by iso-osmotic salt solutions suggested that the...

Cellulose is a renewable and biodegradable natural biopolymersynthesized mainly in plants such as wood, bark, cotton fiber, green algae, some bacteria and animals. Pre-treatment processes such as alkaline purification and enzymatic purification are applied in order to create a proper sorption characteristic of regenerated cellulose fiber. Prior to the application of cellulose/cellulose derivati...

The purpose of this study is to improve the thermo-stabilty of alkaline protease produced by Bacillus sp. using rice bran during solid state fermentation. After partial purification of alkaline protease, various parameters namely effect of temperature and metal ions on enzyme activity was investigated. The suitable temperature for alkaline protease activity was observed at 50°C. Among various m...

Alkaline prorcasex are of considcruhlc interest in view of their activity and stability at alkaline pll. This review describes the protcascs that can resist extreme alkaline environments produced by a wide range of ulk alophilic microorg anisms. Different isol.uion methods which enable the screening and selection of promising organisms 1'01' industrial production are discussed. The various nutr...