BACKGROUND/AIM Alpha- and beta-amanitins are the main toxins of the poisonous Amanita phalloides mushroom. Although there are many studies available concerning alpha-amanitin, there are limited data about beta-amanitin in the literature. Therefore, this study is aimed at comparing the toxic effects of alpha- and beta-amanitin on the MCF-7 cell line. MATERIALS AND METHODS The alpha- and beta-a...

Amanita phalloides, Amanitins, Phalloidin, Mushroom Poisoning, Chromatography A fast, sensitive high performance thin-layer chromatographic method for the determination of a-, ß-, and y-amanitin in crude, methanolic extracts of Amanita phalloides is described. The limit of detection is 50 ng of each amanitin. With this method amanitin was determined in 24 pooled samples of Amanita phalloides, c...

A total of 138 samples o f higher fungi, representing 35 species (15 belonging to the genus Amanita), were analysed for the trace element bromine using spectrophotom etric and gas chromatographic methods. High concentrations o f bromine, up to 100 m g/kg on dry weight, were encountered in Amanitaceae, especially in members o f the subsections Phalloideae and Eu-Amanita, whereas in other fungi t...

Among the violently poisonous species of Amanita, none has received more attention than A. phalloides (Fr.) Secr. By 1959, five related cyclopeptidic toxins had been extracted from this mushroom and named phalloidin, phalloin, and a-, j3-, and y-amanitin. T. Wieland and 0. Wieland (Pharmacol. Rev. 11:87, 1959) reviewed the chemistry and toxicology of these compounds. A sixth toxin, phallacidin,...

By the addition of phalloidin, an oligopeptide of Amanita phalloides, to platelet-rich plasma, an increase of its transparency could be observed. The rate of increase of transparency was slow but the maximal intensity corresponded to that of ADP aggregation. Morphologically, many microfilaments were formed in the cytoplasm. But the figures were different from those in platelets in ADP aggregation.

The deadly poisonous Amanita phalloides is common along the west coast of North America. Death cap mushrooms are especially abundant in habitats around the San Francisco Bay, California, but the species grows as far south as Los Angeles County and north to Vancouver Island, Canada. At different times, various authors have considered the species as either native or introduced, and the question o...

It has recently been shown by one of us (W. W. Ford’) that the poisons of the fungus Amanita phalloides, the variety responsible for the great majority of deaths from mushroom intoxication, belong to the group of bacterial toxins. They were thus classified in virtue of their causing characteristic lesions in animals after a definite latent period, and because an immunity may be established towa...

It has recently been shown by one of us (W. W. Ford’) that the poisons of the fungus Amanita phalloides, the variety responsible for the great majority of deaths from mushroom intoxication, belong to the group of bacterial toxins. They were thus classified in virtue of their causing characteristic lesions in animals after a definite latent period, and because an immunity may be established towa...