the aim of this study was to prepare a new formula of leishmania major (l. major) crude antigen and evaluate its effect on immune system. for this purpose l. major promastigotes were cultured, harvested, washed, and resuspended in physiologic saline and the suspensions were dispersed in five equal batches. 0.1 ml of various doses of the cocktail antigen were injected intradermaly in three group...

visceral leishmaniasis (vl) is a zoonotic disease caused by leishmania species. dogs are considered to be the main reservoir of vl. a number of methods and antigen-based assays are used for the diagnosis of leishmaniasis. however, currently available methods are mainly based on direct examination of tissues for the presence of parasites, which is highly invasive. a variety of serological tests ...

The insolubility of antigen-antibody aggregates formed at most proportions of reactants has prevented physicochemical study of these systems of interacting proteins under conditions in which they remain associated. Only when the antigen is present in great excess and soluble complexes are formed have the products been examined by ultracentrifugation (14)) moving boundary electrophoresis (l-3), ...

INTRODUCTION To determine the immunostimulatory potential of crude extracts of Warburgia ugandensis subsp. ugandensis with a soluble leishmanial antigen in vaccinating BALB/c mice. METHODS Seventy two female BALB/c mice were randomly assigned into six groups. The mice were vaccinated with soluble leishmania antigens (SLA) alone, hexane, ethyl acetate, and dichloromethane extract co-administer...

The aim of the study was to evaluate the concentrations of a soluble form of APO-1/Fas antigen (sFas, CD95) and a soluble Ligand for APO-1/Fas antigen (sCD95L, sFasL) in supernatants from CaSki and HeLa cell line cultures after the incubation with All-trans-retinoic acid. HPV-16 and HPV18 - positive cell lines were cultivated with All-trans-retinoic acid in concentrations of 1 x 10(-6) M/L and ...

background: heat shock proteins (hsp) are highly conserved molecules with many immunological functions. they are highly immunogenic with important role in cancer immunotherapy and in vaccine development against infectious diseases. as adjuvant, hsp can augment the immunogenicity of weak antigens and can stimulate antigen presenting cells. although vaccines have been successful for many infectio...

objective(s): iscomatrix vaccines have now been shown to induce strong antigen-specific cellular or humoral immune responses to a broad range of antigens of viral, bacterial, parasite or tumor. in the present study, we investigated the role of iscomatrix charge in induction of a th1 type of immune response and protection against leishmania major infection in balb/c mice.  materials and methods:...

The potential of Leishmania major culture-derived soluble exogenous antigens (SEAgs) to induce a protective response in susceptible BALB/c mice challenged with L. major promastigotes was investigated. Groups of BALB/c mice were immunized with L. major SEAgs alone, L. major SEAgs coadministered with either alum (aluminum hydroxide gel) or recombinant murine interleukin-12 (rmIL-12), L. major SEA...

The synthesis and transport of the soluble antigen of influenza A virus were traced by immunofluorescent staining with human convalescent sera during abortive and complete infection of hamster BHK21 ceils and mouse L ceils. Times of synthesis in the nucleus, transport to the cytoplasm and extrusion by the cell were the same in complete and in abortive infection. The product of abortive infectio...

The synthesis and transport of the soluble antigen of influenza A virus were traced by immunofluorescent staining with human convalescent sera during abortive and complete infection of hamster BHK21 ceils and mouse L ceils. Times of synthesis in the nucleus, transport to the cytoplasm and extrusion by the cell were the same in complete and in abortive infection. The product of abortive infectio...