نتایج جستجو برای: ammonia mediated method
تعداد نتایج: 2042729 فیلتر نتایج به سال:
Hyperammonemia is a metabolic condition characterized by elevated levels of ammonia and a common event in acute liver injury/failure and chronic liver disease. Even though hepatic ammonia levels are potential predictive factors of patient outcome, easy and inexpensive methods aiming at the detection of liver ammonia accumulation in the clinical setting remain unavailable. Thus, herein we have d...
Addition of ammonia or urea to the combustion zone of a boiler has been shown to reduce the formation (and thus emissions) of oxides of nitrogen. However, under certain conditions, some of the ammonia will “slip” by and may be emitted to the atmosphere. Several states are interested in monitoring such ammonia “slip” emissions and other sources of ammonia. Industries are interested in using ammo...
A coulometric method is described for the determination of ammonia resulting from micro-Kjeldahl digestion of protein samples. The method has been applied to the direct titration of the ammonia in the digests. Routine titration of the ammonia resulting from lOsl. serum samples is described; the method has been applied to 1 -JLI. samples. Results are compared with standard macroand micro-Kjeldah...
Ammonia has been suggested as a carbon-free hydrogen source, but a convenient method for producing hydrogen from ammonia with rapid initiation has not been developed. Ideally, this method would require no external energy input. We demonstrate hydrogen production by exposing ammonia and O2 at room temperature to an acidic RuO2/γ-Al2O3 catalyst. Because adsorption of ammonia onto the catalyst is ...
A method is described for preservation of blood for delayed determinations of ammonia by rapid freezing of the samples in a dry ice and acetone solution followed by storage of the specimens in the frozen state. The method was evaluated by first establishing the reproducibility of the chemical method used for blood ammonia analysis in 714 consecutive samples of dog blood which were analyzed in d...
Glutamine synthetase (GS) catalyzes the recycling of NH4 (+) with glutamate to form glutamine. GS is highly expressed in the renal proximal tubule (PT), suggesting ammonia recycling via GS could decrease net ammoniagenesis and thereby limit ammonia available for net acid excretion. The purpose of the present study was to determine the role of PT GS in ammonia metabolism under basal conditions a...
A new continuous-flow automated enzymatic method suitable for the direct determination of creatinine in physiological samples is described. The proposed system utilizes an on-line gas predialysis unit in conjunction with a flow-through enzyme reactor coil and a potentiometric ammonia detector. The enzyme reactor contains immobilized creatinine iminohydrolase (EC 3.5.4.21) which converts creatin...
We modified the Hyland Ammonia kit for plasma to measure blood ammonia from which the erythrocyte ammonia is calculated. Our modified method gave good recoveries and its precision based on replicate assays was excellent (CV less than 3.0%). The within-day and day-to-day precision was determined from pooled blood and aqueous ammonia solution respectively. The precision calculated from duplicate ...
This study was accomplished to examine the efficiency of natural zeolite in preventing acute toxicity of total ammonia to Huso huso. The study was performed using Water Static Method in 96 hours. Fish averaged 46 ? 5 g in weight and 22 ? 4 cm in total length were exposed to four different concentrations (15, 30, 50, 75 mgL-1) of ammonia and a group was considered as control. Under stable condit...
Relative to the gills, the mechanisms by which the kidney contributes to ammonia and acid-base homeostasis in fish are poorly understood. Goldfish were exposed to a low pH environment (pH 4.0, 48 h), which induced a characteristic metabolic acidosis and an increase in total plasma [ammonia] but reduced plasma ammonia partial pressure (PNH3). In the kidney tissue, total ammonia, lactate and intr...
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