The present study utilized different methods for purification of alkaline protease by using ammonium sulphate and acetone. Ammonium sulphate was found the best purifying agent and gave 109.09 fold purification of protease with 49.7% yield at 60% saturation. Dialysis was performed by using phosphate and Tris-HCl buffer and phosphate buffer was optimized to good dialyzing agent at pH 8.0.

Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were com...

all desalination methods which are currently used have limitations. a newly designed instrument by zare a.m. was tested and compared with currently used method in razi vaccine and serum research institute(rvsri). samples of anti snake venom for desalination was provided by department of anti-serum, rvsri. parameters included in this study were time and water consumption during desalination proc...

The phase transitions and hygroscopic growth of two humic acid aerosols (Aldrich sodium salt and Leonardite Standard (IHSS)) and their mixtures with ammonium sulphate have been investigated using a combination of two techniques, Fourier transform infra-red (FTIR) spectroscopy and tandem differential mobility analysis (TDMA). A growth factor of 1.16 at 85% relative humidity (RH ) was found for t...

Corynebacterium glutamicum is an industrially important microorganism used for amino acids production. The present work describes the production of L-lysine from Corynebacterium glutamicum IIB-C9 through submerged fermentation process. Fifteen fermentation media were screened in this study for L-lysine production. Out of these fifteen media, FM:13 was found a best one for maximum production of ...

Carboxymethylcellulases (CMCases) of Arachniotus citrinus were produced under solid state fermentation on different carbon sources including wheat bran, corn cob, alkali treated corn cob and mixture of wheat bran and alkali treated corn cob at 30C for 15 days. In this case, mixture of wheat bran and corn cob was observed to be the best carbon source as maximum specific activity (4.2 U mg protei...

The structural and electrical properties of polyaniline-cobalt sulphate nanocomposite prepared by chemical method.Polyaniline-cobalt sulphate nanocomposites were synthesized by the cobalt sulphate and ammonium persulphate and aniline.This paper reports on chemical technique using for synthesis of cobalt nanoparticles in the composite bridge is aniline. Electrical conductivity of polyaniline wit...

The structural and electrical properties of polyaniline-cobalt sulphate nanocomposite prepared by chemical method.Polyaniline-cobalt sulphate nanocomposites were synthesized by the cobalt sulphate and ammonium persulphate and aniline.This paper reports on chemical technique using for synthesis of cobalt nanoparticles in the composite bridge is aniline. Electrical conductivity of polyaniline wit...

egg-laying hens were immunized with the echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. the chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (peg) and ammonium sulphate precipitation method, chloroform extraction and the lithium sulphate precipitation method. these methods were com...

The aerosol chamber AIDA was used as a moderate expansion cloud chamber with cooling rates at the onset of ice nucleation between –1.3 and –3.0 K min−1 to investigate the nucleation and growth of ice crystals in sulphuric acid, ammonium sulphate, and mineral dust aerosols at temperatures between 196 and 224 K. Supercooled sulphuric acid droplets with mean diameters of about 0.2 to 0.3 μm nuclea...