PURPOSE Suturing amniotic membrane to cornea during surgery is time consuming, and sutures may further damage the eye. The authors introduce a novel sutureless, light-activated technique that securely attaches amnion to cornea through protein-protein crosslinks. METHODS Cryopreserved human amniotic membrane, stained with Rose Bengal (RB), was placed over a full-thickness wound in deepithelial...

In this study we aimed to set up an in vitro culture of the rabbit amnion in order to support in vivo fetal membrane healing capacity following fetoscopy. Fetal membranes were collected from a mid-gestational rabbit, and cultured on collagen support material for 14 days. 34 rabbits at 22-23 days gestational age (GA) underwent fetoscopy. The entry site was randomly allocated to 4 closure techniq...

background different methods for dressing of donor site of skin graft in burn patients have similarly pain, limitation of mobility of donor site and local complications such as infection and scar. amniotic membrane has used for improvement of healing in some wounds. accordingly in this study amnion was used as biologic dressing for donor site of skin graft to evaluate it’s efficacy in improveme...

Fetal membranes usually rupture during the process of labor. Premature fetal membrane rupture occurs not infrequently and is associated with significant fetal and maternal morbidity. The mechanisms of normal and pathologic fetal membrane rupture are not well understood. We have examined structural and biochemical changes in the rat amnion as labor approaches in order to characterize this proces...

Tracing the precise developmental origin of amnion and amnion-derived stem cells is still challenging and depends chiefly on analyzing powerful genetic model amniotes like mouse. Profound understanding of the fundamental differences in amnion development in both the disc-shaped primate and human embryo and the cup-shaped mouse embryo is pivotal in particular when sampling amniotic membrane from...

A case series of five patients with a total of six chronic non-healing wounds (>30 day duration) were non-randomly selected to evaluate the performance, safety and handling properties of dehydrated human amnion/chorion membrane allograft, an amniotic membrane scaffolding product. The patients had lower extremity wounds that had previously failed standard of care within a university outpatient/i...

Background: Burns are among the most common traumas in developing countries, which consume large amounts of medical resources. It is important to find an appropriate material for dressing of burn wounds that improves healing and is readily available, easily applicable, and economical. Methods: In a single-blind randomized controlled clinical trial from March to October 2006, 211 patients with l...

The amniotic membrane is a tissue of fetal origin and is composed of three major layers: a single epithelial layer, a thick basement membrane, and an avascular mesenchyme [Fernandes et al., 2005]. There are no nerves, muscles or lymphatics in the amnion. The amniotic membrane is derived from the inner cell mass in the blastocyst and is adjacent to the trophoblast cells and lines the amniotic ca...

background: amniotic membrane derived stem cells (amscs) have considerable advantages to use in regenerative medicine and their anti- inflammatory effects, growth factor secretion and differentiation potential make them suitable candidates for stem cell therapy of nervous system. the developing and neonatal brain contains a spectrum of growth factors to direct development of endogenous and dono...