Amoebic gill disease (AGD) is a significant issue in Atlantic salmon mariculture. Research on the development of treatments or vaccines uses experimental challenges where exposed to amoebae concentrations ranging from 500 5,000/L. However, water N. perurans affected farms are much lower. The lowest concentration previously reported cause AGD was 10/L. Here, we report that as low 0.1/L can AGD. ...