An amperometric sensor based on yttria-stabilized zirconia (YSZ) and an oxidebased sensing electrode (SE) was examined for the detection of propene. Among the various oxide-based SEs tested, ZnO (+8.5 wt.% Pt) was found to give good sensitivity and selectivity to C3H6 at 600°C. A novel measuring method named “pulsed-potential method” was adopted here to obtain improved sensing characteristics. ...

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear...

The locus coeruleus (LC) harbors a compact group of noradrenergic cell bodies projecting to virtually all parts of the central nervous system. By using combined measurements of amperometry and patch-clamp, quantal vesicle release of noradrenaline (NA) was detected as amperometric spikes, after depolarization of the LC neurons. After a pulse depolarization, the average latency of amperometric sp...

The concentration of a substrate in a solution can be measured using amperometric signals of biosensors: in fact the maximum (steady state) current is measured which is calibrated in the units of concentration. Such a simple method is not applicable in the case of several substrates. In the present paper, the problem of evaluation of concentrations of several substrates is tackled by minimizing...

We report details of an amperometric method for measurement of hydrogen (H2) in the aqueous phase and its application to hydrogen evolution by an alga. Although amperometric measurement of hydrogen is not common, we wondered whether the conventional Clark electrode for measurement of oxygen could be applied to measurement of hydrogen simply by proper choice of voltage. Initial tests were encour...

Gallium ultramicroelectrodes for amperometric measurements in scanning electrochemical microscopy were fabricated by introduction of liquid Ga into drawn glass micropipets. Cyclic voltammetry of Ru(NH(3))(6)(3+) and the use of this species for SECM imaging is described. Double-barrel micropipet tips with a Ga amperometric electrode and an ion-selective (K(+)) potentiometric probe can also be co...

We examined the relative merits of direct-current, pulse, and differential pulse amperometry at a flow-through, glassy-carbon electrode used in monitoring column chromatography. We give a rapid, simple procedure for determining theophylline (1,3-dimethylxanthine) in 20--100 microliter of blood serum. Interference by 1,7-dimethylxanthine, a metabolite of caffeine, is compared for photometric and...

I would like to model and simulate 3D label free Nano Amperometric Biosensor for drug delivery and detection of cancerous cells. An Amperometric biosensor is one which has a biological element and a transducer. The analysis to whom the transduction to take place is referred to as analyte. The biological element I use in my work is enzyme, it can be even a living cell, DNA and proteins. Enzyme w...

A disposable amperometric immunosensor was studied for the rapid detection of Vibrio cholerae (V. cholerae), the causative agent of cholera, employing an indirect sandwich enzyme linked immunosorbent assay (ELISA) principle. Screen-printed electrodes (SPEs) were fabricated (by using commercial and homemade carbon inks), electrochemically characterized and the assay conditions were optimized for...

This amperometric technique for the determination of enzyme activity is based on detecting a decrease in the concentration of the NADH co-factor of the enzyme reaction. A glassy carbon electrode, modified by adsorption of Mg2+ and NADH, is used to measure the anodic peak current that corresponds to the oxidation of NADH. We found no significant difference between the enzyme activity of lactate ...