a flow injection method is reported for the determination of ascorbic acid based on amperometric detection, using a cobalt(ii) phthalocyanine (copc) modified graphite-epoxy electrode. the amperometric respons was evaluated with regard to ph, ionic strength of the electrolyte, flow rate of the carrier solution, injected sample volume and conditioning time of the electrode. the limit of detection...

During exocytosis, the fusion pore expands to allow release of neurotransmitters and hormones to the extracellular space. To understand the process of synaptic transmission, it is of outstanding importance to know the properties of the fusion pore and how these properties affect the release process. Many proteins have been implicated in vesicle fusion; however, there is little evidence for prot...

In recent years, constant applied potential molecular dynamics has allowed to study the structure and of electrochemical double-layer a large variety nanoscale capacitors. Nevertheless it remained impossible simulate polarized electrodes at fixed total charge. Here we show that combining electrode with finite electric displacement fills this gap by allowing open circuit conditions. The method c...

We aim to develop an in situ microfluidic biosensor based on laccase from Trametes pubescens with flow-injection and amperometry as the transducer method. The enzyme was directly immobilized by potential step chronoamperometry, and the immobilization was studied using cyclic voltammetry and electrochemical impedance spectroscopy. The electrode response by amperometry was probed using ABTS and s...

The present paper aims to miniaturize a graphite-epoxy and synthetic zeolite-modified graphite-epoxy composite macroelectrode as a quasi-microelectrode aiming in vitro and also, envisaging in vivo simultaneous electrochemical detection of dopamine (DA) and ascorbic acid (AA) neurotransmitters, or DA detection in the presence of AA. The electrochemical behavior and the response of the designed m...

The fabrication of ultrathin-ring electrodes with a diameter of 2 mm and a thickness of 100 nm is established. The ultrathin-ring electrodes provide a large density of pseudo-steady-state currents, and realize pseudo-steady-state amperometry under quiescent conditions without a Faraday cage. Under the limiting current conditions, the current response at the ultrathin-ring electrode can be well ...

In this article, we have summarized the recent important results related to electrochemical detection of vesicular exocytosis by amperometry with microelectrodes over past three years. fascinating scientific field that began 40 years ago, historical carbon fiber method still continues be used address biological questions pioneered groups but also other research thus showing has become an indisp...