Somatic embryos and rooted plantlets have been regenerated from light-initiated embryogenic callus derived from mature embryos of Picea abies stored seeds. Under a 16 hour photoperiod, mature zygotic embryos were cultured on a modified half-strength Murashige-Skoog medium without NH4NO3 and supplemented with 5 mM glutamine, 4.5 pM N6-benzyladenine (BA) and 10.7 PM naphthalene-acetic acid (NAA) ...

Embryogenic callus was induced from culturing immature embryos of three barley cvs. Dissa, Golden Promise and Igri. Maximum embryogenic callus formation was achieved by culturing immature embryos on CC and LS medium containing 2 mg/litre and 2.5 mg/litre 2,4dichlorophenoxyacetic acid (2,4-D) respectively. Embryogenic calli were frequently produced distinguished somatic embryos on these medium. ...

Brachypodium distachyon L. Beauv. (Brachypodium) is a species that has become an excellent model system for gaining a better understanding of various areas of grass biology and improving plant breeding. Although there are some studies of an in vitro Brachypodium culture including somatic embryogenesis, detailed knowledge of the composition of the main cell wall components in the embryogenic cal...

background: sugarcane is a monocotyledonous crop that is cultivated in the tropical and subtropical regions of the world. one of the most important criteria, influencing the efficiency of the sugarcane transformation is known to be related to physical and biological factors during the transformation procedure. objectives: the objective of this research was to study the response of callus induc...

Single cells were mechanically isolated from leaf-derived callus of mature Juniperus oxycedrus L. These cells divided and gave rise to callus when plated on medium containing growth regulators. Best plating efficiency was obtained on a modified Schenk and Hildebrandt medium supplemented with 0.6 micro M 2,4-dichlorophenoxyacetic acid and 100 mg l(-1) casein hydrolyzate. Although single-cell-der...

In this paper, we describe the transformation of regenerable maize tissues by electroporation. In many maize lines, immature zygotic embryos can give rise to embryogenic callus cultures from which plants can be regenerated. Immature zygotic embryos or embryogenic type I calli were wounded either enzymatically or mechanically and subsequently electroporated with a chimeric gene encoding neomycin...

The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to ob...

During the last decade, considerable effort has been expended in developing an efficient transformation system for sugarcane. Most protocols employ microprojectile bombardment of gene constructs into embryogenic sugarcane callus. The numbers of transgenic plants produced per bombardment range from 0.1-20 globally, with genotype response and selection regime appearing to be the most important fa...