Local and Long-Range Electrostatic Interactions in Denatured Ribonuclease Sa: Comparing the Denatured Protein with Model Peptides (April 2002) Michael Douglas Daily Department of Biochemistry and Biophysics Texas A&M University Fellows Advisor: Dr. J. Martin Scholtz Department of Biochemistry and Biophysics The folding ol'proteins into three-dimensional, biologically active conformations demand...

The relative activity of lipoprotein lipase (LPL) in different tissues controls the partitioning of lipoprotein-derived fatty acids between sites of fat storage (adipose tissue) and oxidation (heart and skeletal muscle). Here we used a reverse genetic strategy to test the hypothesis that 4 angiopoietin-like proteins (ANGPTL3, -4, -5, and -6) play key roles in triglyceride (TG) metabolism in hum...

Angiopoietin-related growth factor (AGF; or Angptl6) is a liver-derived, circulating factor and is considered to be a regulator of metabolic homeostasis. AGF is capable of counteracting both obesity and obesity-related insulin resistance. However, the target tissues and the molecular mechanisms underlying the antiobesity and antidiabetic actions of AGF have not been completely defined. Using ra...

Obesity is an increasing health problem all over the world. Angiopoietin-related growth factor (AGF, ANGPTL6) a hepatocyte that directly regulates lipid, glucose and energy metabolism independent of angiogenic effects. In this study, we investigated circulating AGF levels in relation to kidney function, type 2 diabetes mellitus insulin resistance. was determined by enzyme-linked immunosorbent a...

Deployment of distributed renewable energy resources is increasing rapidly, which is leading to growing concerns over the impact of distributed power electronics energy converters on grid stability. In general, power electronic coupled systems do not provide frequency or voltage support through feedback compensation. This is leading to changes in utility interconnection requirements for distrib...

Here we reported the use of electropolymerization to achieve the transformation of aqueous hydroquinone to solid-phase polyhydroquinone (PHQ) with pseudocapacitive characteristics, and the application of this redox-active product to shuttle electron transfer in the anode system of a microbial fuel cell (MFC). The microscopic and spectroscopic results showed that the treatment of the graphite fe...

Tumor cell-derived hyaluronidase HYAL-1 degrades hyaluronic acid (HA) into angiogenic fragments (AGF: 10-12 disaccharides). AGF support tumor growth and progression. Urine and tissue HAase/HYAL-1 levels are sensitive markers for high-grade bladder cancer (BCa) and its metastasis. In preclinical models of BCa, we evaluated whether o-sulfated AGF (sHA-F) inhibits HAase activity and has antitumor ...

The unfolding of ribonuclease, lysozyme, a-chymotrypsin, and goat P-lactoglobulin by urea and guanidine hydrochloride (GmHCl) has been followed with the use of optical rotation measurements. Urea denaturation leads to a more negative rotation for each protein than does GmHCl denaturation, but the concentration dependence is such that the rotations are almost identical in the absence of denatura...

Content General methods .............................................................................................................................................. S1 Apparatus ......................................................................................................................................................... S2 Abbreviations..................................................