Serum samples from 55 patients with systemic lupus erythematosus (SLE) were selected for the absence of anti-extractable nuclear antigen antibodies after routine immunodiffusion tests. These sera were immunoblotted for anti-Sm and anti-RNP antibodies on a HeLa cell nuclear extract. Ten (18%) were negative and 45 (82%) produced complex patterns: 10 (18%) suggestive of anti-Sm, three (5%) anti-RN...

The presence of H-2 gene products on mixed leukocyte reaction (MLR) supressor factor was investigated by passage of MLR-suppressor factor (SF) over solid immunoadsorbents prepared with various anti-H-2 subregion sera. Antisera with specificity for all or certain I subregion determinants removed or significantly reduced suppressor activity; adsorption was not consistent with K or D region specif...

A method is described for the automated quantitation of the anti-D content of sera. This procedure is far more reproducible than current manual techniques. The use of the International Standard anti-D typing serum in the calibration of a working standard enables results to be expressed in mug/ml of antibody protein.

In the mouse the H-2 complex is divided into a number of regions consisting of K, I, S, G, and D, (1). Of these, the I region has proven to be of part icular interest as it contains genes which effect a number of diverse biological phenomena. Included in these are the histocompatibility ( H ) I region genes and it has been of interest to compare the different K, I, and D (H) genes. Each of thes...

Commercially prepared ABO blood-typing antisera have been tested for their ability to bind to murine laminin and their cytotoxic effects upon high and low metastatic variants of a murine fibrosarcoma. Previous studies have shown that alpha-D-galactopyranosyl end-groups comprise the major antigenic determinants on type B erythrocytes and that these same end-groups are present on murine laminin p...

Abstract Eritrea is an East African multiethnic country with intermediate endemicity for hepatitis B. Our aim was to establish the most prevalent genotypes of B virus (HBV) among patients liver disease. A total 293 Eritrean disease who were surface antigen (HBsAg) positive enrolled. All sera tested transaminases, HBV DNA viral load, and seromarkers including HBsAg, anti-HBcAb (total), HBeAg, an...

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The purpose of this study was to evaluate the protective efficacy a vaccine consisting recombinant Neospora caninum-cyclophilin (NcCyP) and -profilin (NcPro) in sheep. At 42 d 21 prior mating, adult Dorset ewes were immunized with rNcCyP-rNcPro (Group 1) or co-purifying non-recombinant (NR) control 2). 90 days post-mating, all challenged by intravenous injection 106 Nesopora caninum Illinois ta...

Present concepts of the maturation of a B-lymphocytic clone involve progressive changes in cellular morphology and mode of immunoglobulin (Ig) production. The ancestral small lymphocyte synthesizes Ig for insertion into its plasma membrane, while those of its descendants destined to produce humoral antibody show first a superimposed synthesis of Ig for export and finally a heavy export with lit...