phage elisa is a common method to confirm binding of obtained phages from phage display technique to related antigens. enzyme-conjugated antibody directed against the major capsid protein (pviii) or enzyme-conjugated secondary antibody against the primary antibody is used as a detection system in phage elisa. we have suggested to express an acid phosphatase (sapm) enzyme on m13 pix minor coat p...

introduction: determination of the efficacy of crude entamoeba histolytica extract and its fractions (fi,fii & fiii) in antiamoebic antibody detection by elisa. material and methods: entamoeba histolytica nih:200 was cultured axenically and different fractionsof its crude amoebic extract, i.e. fi, fii & fiii, were obtained by column chromatography. efficacy of crudeand fractioned antigens for a...

mycoplasma genitalium (m.genitalium) is a sexually transmitted pathogen. detection of this microorganism in clinical specimens by culture is rather difficult and time consuming. the aim of this study was to produce polyclonal antibody against a synthetic peptide from p110 protein of m.genitalium in order to develop a diagnostic tool for detection of this microorganism in clinical specimens. a s...

Background and Aims: Influenza is an acute respiratory illness that is caused by a virus belonging to Orthomyxoviridae family. This virus spreads rapidly every year in cold season and leads to morbidities and mortalities especially in adults and children, which causes billions of dollars of economic losses. Accordingly, development of a rapid, sensitive and inexpensive laboratory diagnosis base...

the aim of this study was to investigate the hemagglutination properties of caprine herpesvirus-1(cphv-1) and their application for cphv-1 antigen detection and evaluation of the dynamics of postinfectious/vaccine antibody production. cphv-1 hemagglutination activity was proved only with mouseerythrocytes, pbs and saline solutions at 4°c and 22°c. in vaccinated animals, goats and bucks withresp...

With the aim of the production of human factor VIII antigen and its corresponding antibody an epitope coding fragment of the light-chain of hFVIII, fused to a His6-tag, was isolated and over-expressed in Escherichia coli. The over-expressed hFVIII-epitope containing peptide was confirmed by its reaction with a rabbit serum directed against native hFVIII as well as antiHis6-tag antibody. An expr...

Abstract BACKGROUND: Diagnosis of Echinococcus granulosus in the definitive host particularly in dog is the significant complication in the endemic area. OBJECTIVES: The aim of this study is serological detection of E. granulosus in the infected dogs. METHODS: Dot-ELISA based on the copro-antigen and recombinant EPC1 antigen (rEPC1) for antibody detection was performed. Blood and fecal samples ...