The Mycobacterium tuberculosis 30 kDa major secretory protein (antigen 85B) is the most abundant protein exported by M. tuberculosis, as well as a potent immunoprotective antigen and a leading drug target. A mycolyl transferase of 285 residues, it is closely related to two other mycolyl transferases, each of molecular mass 32 kDa: antigen 85A and antigen 85C. All three catalyze transfer of the ...

Background Bovine tuberculosis (TB) is a disease which constitutes an important economic and zoonotic problem in Latin America. The etiological agent, Mycobacterium bovis, has several antigens described and well characterized. The M. bovis antigen 85B (Ag85B), encoded by fbpB gene, is the bacillus immunodominant antigen and promising target for vaccine and diagnosis development [1]. Ag85B combi...

Direct effects of Helicobacter pylori (H. pylori) on human CD4+ T-cells hamper disentangling a possible bacterial-mediated interference with major histocompatibility complex class II (MHC-II)-dependent antigen presentation to these cells. To overcome this limitation, we employed a previously described assay, which enables assessing human antigen-processing cell function by using murine T-cell h...

BACKGROUND Tuberculosis is the leading cause of death due to bacterial infections worldwide, mainly caused by Mycobacterium tuberculosis. The antigen 85 complex comprises a set of major secreted proteins of M. tuberculosis, which are potential biomarkers for diagnostic. RESULTS In this work, the first human single chain fragment variable (scFv) antibodies specific for the tuberculosis biomark...

T helper 1 cells play a major role in protective immunity against mycobacterial pathogens. Since the antigen (Ag) specificity of CD4(+) human T cells is strongly controlled by HLA class II polymorphism, the immunogenic potential of candidate Ags needs to be defined in the context of HLA polymorphism. We have taken advantage of class II-deficient (Ab0) mice, transgenic for either HLA-DRA/B1*0301...

Abstract Tuberculosis (TB), as a common infectious disease, still remains severe challenge to public health. Due the unsatisfied clinical needs of currently available diagnostic vehicles, it is desired establish new approach for universally detecting Mycobacterium tuberculosis. Herein, we designed real-time recombinase polymerase amplification (RPA) technology identifying M. tuberculosis within...

Identification and characterization of serologically active mycobacterial antigens are prerequisites for the development of diagnostic reagents. We examined the humoral immune responses of active tuberculosis (TB) patients against Triton-soluble proteins extracted from Mycobacterium tuberculosis by immunoblotting. A 29-kDa protein reacted with immunoglobulin M (IgM) in the pooled sera of the pa...